脂多糖对人牙周韧带细胞中MT1-MMP表达的影响
刘念;曹颖光;朱光勋
【期刊名称】《临床口腔医学杂志》 【年(卷),期】2018(034)002
【摘要】Objective:To investigate the effects of lipopolysaccharide in graded concentration and stimulating time on MT1-MMP expression in human periodontal ligament cells.Methods:Cultured hPDLCs were stimulated with lipopolysaccha-ride in concentration and time gradient respectively.Western Blot and quantitative real-time PCR were employed to
determine
MT1-MMP
expressions
at
protein
and
gene
level.Results:LPS stimulation reduced the expressions of MT1-MMP protein and mRNA,and the regulatory effects of LPS exhibited a certain concentration and time dependence(P<0.05).Conclusion:MT1-MMP expressions were significantly enhanced in LPS-stimulated hPDLCs,MT1-MMP may be involved in inflammatory reactions of periodontal tissues.%目的:探索在不同浓度及作用时间的脂多糖刺激下MT1-MMP在人牙周韧带细胞中的表达.方法:体外培养人牙周韧带细胞,取4~7代细胞分别以脂多糖梯度浓度和梯度时间进行干预,利用Western Blot和实时定量荧光PCR检测细胞中MT1-MMP在蛋白及基因水平上的表达.结果:Western Blot结果示脂多糖刺激下人牙周韧带细胞中MT1-MMP的蛋白表达强度升高,并在一定范围内呈现浓度和时间依赖性(P<0.05);定量PCR结果显示MT1-MMP mRNA的表达变化趋势与Western Blot结果相一致(P<0.05).结论:脂多糖刺激下人牙周韧带细胞
脂多糖对人牙周韧带细胞中MT1-MMP表达的影响
