HPLC测定L-肌肽中L-组氨酸及其中间体的含量
刘荣磊;陈玉荣;杨海霞;张治云
【期刊名称】《食品与药品》 【年(卷),期】2018(020)002
【摘要】目的 建立高效液相色谱法(HPLC)测定L-肌肽中L-组氨酸及其中间体含量的方法.方法 采用Atlantis HILIC Silica(150 mm×4.6 mm,5 μm)色谱柱,柱温35 ℃,流动相为0.01 mol/L醋酸铵溶液(加冰醋酸调pH4.0)-乙腈(28:72),流速1.0 ml/min,检测波长220 nm.结果 L-组氨酸在0.5093～10.1856 μg/ml范围内与峰面积有良好线性关系(r=0.9995),平均回收率为99.30 ％(RSD=0.99 ％);中间体在0.4810～9.6206 μg/ml范围内与峰面积有良好线性关系(r=0.9991),平均回收率为98.99 ％(RSD=1.85 ％).结论 方法专属性强、重现性好、灵敏度高,可用于L-肌肽中L-组氨酸及中间体含量的测定.%Objective To establish a method for determination of L-histidine and intermediate in L-carnosine by HPLC. Methods An Atlantis HILIC Silica column (150 mm×4.6 mm, 5 μm) with DAD detecter was adopted, using 0.01 mol/L ammonium acetate (adjust pH to 4.0 with glacial acetic acid)-acetonitrile (28:72, v/v) as mobile phase. The detection wavelength was 220 nm, the flow rate was 1.0 ml/min and the column temperature was 35 ℃. Results A good linear relationship was obtained in the concentration rang of 0.5093-10.1856 μg/ml for histidine, with the correlation coefficient of 0.9995. The average recovery was 99.30 ％ (n=6), with RSD of 0.99 ％. There was a good linear relationship whithin
HPLC测定L-肌肽中L-组氨酸及其中间体的含量
