Protective effect ofAmorphophallus campanulatus (Roxb.) Blume. tuber against thioacetamide

Protective effect ofAmorphophallus campanulatus

(Roxb.) Blume. tuber against thioacetamide

induced oxidative stress in rats

Puthuparampil Nazarudeen Ansil;Anand Nitha;Pallara Janardhanan Wills;Vahab Jazaira;Mukalel Sankunni Latha

【期刊名称】《亚太热带医药杂志（英文版）》 【年(卷),期】2011(000)011

【摘要】Objective:To identify the phytochemical constituents ofAmorphophallus campanulatus (A. campanulatus) tuber and to evaluate its antioxidant potential throughin vitro andin vivo models. Methods: Phytochemical screening andin vitro antioxidant activities ofA. campanulatus tuber n-hexane extract (ACHE) and methanolic extract (ACME) were evaluated using DPPH, hydroxyl radical, reducing power and total antioxidant capacity assays. The total phenolic and flavonoid contents were also investigated. The protective potential of two different doses ofACME (125 and250 mg/kg) was also evaluated against thioacetamide (TAA) induced oxidative stress in rats. Silymarin used as a standard drug control. Hepatotoxicity was assessed by quantifying the serum

levels

of

aspartate

aminotransferase

(AST),

alanine

aminotransferase (ALT), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH). The antioxidant potential ofACME were also evaluated by the estimation of catalase (CAT), glutathione peroxidase