结缔组织铺片脂肪组织油红染色在组织学实验教学中的应
用
王俊梅;平苏宁§;汪泓;黄锦桃;陈大堤;李朝红
【期刊名称】《中国组织化学与细胞化学杂志》 【年(卷),期】2014(000)004
【摘要】Objective To establish a method to detect adipose tissue in the connective tissue for ex-perimental teaching of histology and embryology of medical undergraduates based on the traditional con-nective tissue spread .Methods After loose connective tissue spread adipose tissue was stained with oil red-toluidine blue-eosin triple staining ,and observed by the optic microscopy .Results The fat droplets in the adipose cells of the connective tissue with oil red staining represented red color .The adipose cells located in the center of adipose tissue evenly filled with red lipid droplets ,while the adipose cells located in the pe-riphery of adipose tissue demonstrated no fat droplets ,indicating different phenotypes of fat cells existed in adipose tissue .Toluidine blue staining demonstrated the clustered mast cells in the loose connective tis-sue in blue-colored cytoplasma ,and light nuclei .The other cells and collagen fibers in the connective tis-sue stained by eosin represented pink .Conclusion The oil red-toluidine blue-eosin
triply
staining
can
clearly
represent
more
cell
types ,e .g .mast cells ,and adipose cells in the loose connective tissue
结缔组织铺片脂肪组织油红染色在组织学实验教学中的应用
