The Susceptibility of Primary Dermis Fibroblasts from the Chinese Tree Shrew to Human Cyto

The Susceptibility of Primary Dermis Fibroblasts

from the Chinese Tree Shrew to Human

Cytomegalovirus Infection

Shu-Wei Dong[1];Ling-Shuai Jiao[1];Ming Yang[1];Ying-Liang Duan[2];Yi-Bo Chen[1];Fei Zhao[3];A-Mei Zhang[1];Li Liu[1];Min-Hua Luo[3];Xue-Shan Xia[1];

【期刊名称】《中国病毒学：英文版》 【年(卷),期】2019(034)003

【摘要】As a universal pathogen leading to neonatal defects and transplant failure,human cytomegalovirus(HCMV)has strict species specificity and this has prevented the development of a suitable animal model for the pathogenesis study.The mechanism of cross-species barrier remains elusive and there are so far no non-human cell culture models that support HCMV replication.The Chinese tree shrew(Tupaia belangeri chinensis)is a small laboratory animal and evolutionary closely related with primates.We investigated the susceptibility of primary tree shrew dermis fibroblasts(TSDF)to HCMV infection.Infection with a GFP-expressing HCMV virus resulted in green fluorescence in infected cells with the expression of IE1,UL44 and pp28.The titers of cell-free viruses reached 10^3 PFU/mL at 96 hpi,compared to titers of 10^4 PFU/mL observed in primary human foreskin fibroblasts.Our results suggested that TSDF was semi-permissive for HCMV infection.The TSDF model could be further used to investigate key factors influencing cross-