灵芝粗多糖对二乙基亚硝胺诱导的小鼠肝脏组织形态及原
癌基因和抑癌基因的影响
冯嫣;王家东;冯翠萍;常明昌;程红艳;孟俊龙
【期刊名称】《食用菌学报》 【年(卷),期】2014(000)001
【摘要】Sixty healthy,four week-old,mice were randomly divided into six groups
(female∶male=1∶1
)as
follows;normal
control,diethylnitrosamine (DEN)model group,Ganoderma lucidum polysaccharide (GP) group,and low (DEN + 50 mg/kg·d),median (DEN+100 mg/kg·d)and high (DEN+200 mg/kg·d)GP dose groups.After 45 days,liver tissue morphology was observed using hematoxylin-eosin staining,and proto-oncogene (ras and H-myc)and tumor suppressor gene (p53 and Rb1)mRNA expression levels were determined using fluorescent quantitative real-time polymerase chain reaction (RT-PCR ). Our data demonstrated that GP had the protective function on liver cells of DEN-treated mice,and inhibited to a limited extent the occurrence or development of cancer by regulating the expression of proto-oncogenes and tumor suppressor genes.%采用苏木精-伊红染色法(hematoxylin-eosin staining )和实时荧光定量 PCR(real-time PCR,RT-PCR)法,研究灵芝(Ganoderma lucidum)粗多糖对二乙基亚硝胺(diethylnitrosamine,DEN)诱导的小鼠肝脏组织形态以及原癌基因H-ras和myc 和抑癌基因P53、Rb1 mRNA的影响,结果表明：灵芝粗多糖对 DEN 引
灵芝粗多糖对二乙基亚硝胺诱导的小鼠肝脏组织形态及原癌基因和抑
