二氮嗪预处理对心肌缺血再灌注损伤的保护作用及对连接
蛋白43的影响
陈天平;张乃菊;蔡鑫
【期刊名称】《蚌埠医学院学报》 【年(卷),期】2012(037)009
【摘要】Objective: To observe the protective effect of diazoxide preconditioning on rat myocardial ischemia-reperfusion injury in vivo and observe the effect of diazoxide preconditioning on connexin 43 ( Cx43 ). Methods: Forty-five male SD rats were randomly divided into five groups; sham group, IRI group, IPC group, DPC group and 5-HD group, 9 rats in each group. Myocardial ischemia-reperfusion injury model was established by ligation of left anterior descending coronary artery for 30 minutes, followed by 120 minutes of reperfusion. The left ventricular systolic pressure, left ventricular diastolic pressure and other heart function were measured by Medlab biological signal collecting and processing system. After reperfusion, myocardial enzymes in plasma were tested; and then the expressions of TCx43 and PCx43 of ischemia-reperfusion myocardium were studied by western blot. Results: Compared with IRI group, the levels of CK-MB and LDH were decreased with significant statistical difference in DPC group ( P < 0. 05 - P < 0. 01 ) ; the expression level of Cx43 protein was significantly higher, especially of PCx43(P <0. 01) ;and left ventricular systolic and diastolic
二氮嗪预处理对心肌缺血再灌注损伤的保护作用及对连接蛋白43的影响
