microRNA-18a Promotes Cell Migration and Invasion Through Inhibiting Dicer l Expression in

microRNA-18a Promotes Cell Migration and Invasion Through Inhibiting Dicer l Expression in

Hepatocellular CarcinomaIn Vitro

Xiufen Zhang;Bo Yu;Fuzheng Zhang;Zijian Guo;Lihua Li

【期刊名称】《中国医学科学杂志（英文版）》 【年(卷),期】2017(032)001

【摘要】Objective To investigate the effects of microRNA-18a (miR-18a) on migration and invasion of hepatocellular carcinoma (HCC) cells, and its possible mechanism associated with Dicer l. Methods HepG2 and HepG2.2.15 cells were transfected with miR-18a inhibitor using Lipofectamine. Cell invasion was evaluated by transwell invasion assay, and cell migration was detected by transwell migration and wound-healing assays. Moreover, luciferase reporter assay was used to identify whether Dicer expression was regulated by miR-18a. Real-time RT-PCR and western blot were performed to analyze Dicer 1 expression. In addition, a functional restoration assay was performed to investigate whether miR-18a promotes HCC cell migration and invasion by directly targeting Dicer 1. Results miR-18a inhibitor can suppress the migration and invasion of HCC cells. Furthermore, suppression of Dicer l expression by small interfering RNA essentially abolished the inhibition of cell migration and invasion induced by miR-18a inhibitor, restorating these activities to levels similar to the parental HCC cells. Interestingly,