肾盂输尿管连接部狭窄段缝隙连接蛋白43表达及其临床意
义研究
刘绍华;陈勇;邹音;饶品德;肖强
【期刊名称】《中国实用儿科杂志》 【年(卷),期】2009(024)010
【摘要】目的 探讨小儿先天性肾盂输尿管连接部狭窄段组织缝隙连接蛋白43(Cx43)和Ki-67的表达变化及其与先天性肾积水的发病机制关系.方法 40例病例组标本选自江西省儿童医院2007年6月至2008年8月行经Anderson-Hynes术切除,且临床及病理资料完整的先天性肾积水患儿的肾盂输尿管连接部狭窄段,所有患儿均经影像学及B超检查证实存在肾盂输尿管连接部狭窄和先天性肾积水;对照组标本来自10例肾盂输尿管连接部正常患儿,其中肾母细胞瘤6例,肾发育不良3例,尸检标本1例.应用SP免疫组化方法检测两组标本肾盂输尿管连接部管壁Cx43和Ki-67的表达.结果 病例组和对照组纵、环肌肌间均表达Cx43与Ki-67.病例组Cx43染色阳性细胞数低于对照组,Ki-67染色阳性细胞数高于对照组,差异均有统计学意义(P<0.05).结论 Cx43分布异常导致了先天性肾积水患儿肾盂输尿管连接部狭窄段的细胞增殖和动力传导异常,从而引起或加重先天性肾积水.%Objective To study the expression of gap junction protein connexin 43 and Ki-67 in the stenotic tissue of congenital ureteropelvic junction obstruction in children and the relationship between them and the pathogenesis of con-genital hydronephrosis. Methods Case groups biopsies from forty pyeloplasty specimens were included in the study, which were sectioned through Anderson- Hynes and stained for
connexin 43. These patients were confirmed by imaging and B-mode ultrasonic examination. Ten biopsies with normal ureteropelvic junction including 6 cases of Wilms tumor tissue , 3 cases of renal hypoplasia and one case of normal UPJ tissue specimens were selected as control group. Immu-nohistochemistry methods were used to detect the expression of Cx43 and Ki-67 in ureteropelvic junction wall. Results Gap junction Cx43 and Ki-67 were expressed in case and control groups.They were localized to between the circular muscle and longitudinal muscle. Expression of Cx43 in case group was lower than in control group (P < 0.05). Expres-sion of Ki-67 in case group was significantly higher than control group(P < 0.05), the difference being of statistical sig-nificance (P < 0.05 ).Conclusion The lack of connexin 43 may lead to the abnormal spontaneous dynamic conduction and cell proliferation in ureteropelvic junction stenosis segment. 【总页数】3页(795-797)
【关键词】肾积水;输尿管肾盂连接部;缝隙连接蛋白43;Ki-67;免疫组织化学 【作者】刘绍华;陈勇;邹音;饶品德;肖强
【作者单位】江西省南昌大学研究生院医学部2006级,江西南昌,330006;江西省儿童医院,江西南昌,330006;江西省儿童医院,江西南昌,330006;江西省儿童医院,江西南昌,330006;江西省儿童医院,江西南昌,330006 【正文语种】中文 【中图分类】R72
【文献来源】
https://www.zhangqiaokeyan.com/academic-journal-cn_chinese-journal-practical-pediatrics_thesis/0201236047397.html 【相关文献】
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肾盂输尿管连接部狭窄段缝隙连接蛋白43表达及其临床意义研究
