Experimental cloning of embryos through human-rabbit inter-species nuclear transfer

Experimental cloning of embryos through human-rabbit inter-species nuclear transfer

JI Jingjuan;GUO Tonghang;TONG Xianhong;LUO Lihua;ZHOU Guixiang;FU Yingyun;LIU Yusheng

【期刊名称】《中国高等学校学术文摘·生物学》 【年(卷),期】2007(002)001

【摘要】Therapeutic cloning,which is based on human somatic cell nuclear transfer,is one of our major research objectives.Though inter-species nuclear transfer has been introduced to construct human somatic cell cloned embryos,the effects of type,passage,and preparation method of donor cells on embryo development remain unclear.In our experiment,cloned embryos were reconstructed with different passage and preparation methods of ossocartilaginous cell,skin fibroblast,and cumulus cells.The cumulus cell embryos showed significantly higher development rates than the other two (P＜0.05).The development rate of embryos reconstructed with skin fibroblasts of different passage number and somatic cells of different chilling durations showed no significant difference.Also,fluorescence in situ hybridization (FISH)was conducted to detect nuclear derivation of the embryos.The result showed that the nuclei of the inter-species cloned embryo cells came from human.We conclude that (1)cloned embryos can be constructed through human-rabbit interspecies nuclear transfer;(2)different kinds of