HPLC 测定益坤宁片中阿魏酸的含量
陈美玲;刘琴;张倩;张娟;吴翠;孙艳丽;高晓艳
【期刊名称】《医学信息》 【年(卷),期】2016(029)017
【摘要】Objective To establish yi kun ning of ferulic acid content determination method . Methods Using high performance liquid chromatography (HPLC) method, Phenomenex chromatographic column (250 mm x 4.6 mm, 5μm), mobile phase of acetonitrile - 0.1% phosphoric (20:80),Flow rate 1.0 ml min - 1, column temperature 30 ℃, detection wavelength of 322 nm. Results Puerarin in sample quantity is 5.18 μg/ml~103.6μg/ml range , Show a good linear relationship (r = 0.9993),The recovery was 97.6%, RSD was 1.2% . Conclusion This method is rapid and accurate. The stability of the method, good repeatability,and can be used for yi kun ning slice quality control are analyzed.%目的建立℃坤宁片中阿魏酸含量测定的方法。方法采用高效液相色谱法， Phenomenex 色谱柱(250 mm×4.6 mm，5μm)，流动相为乙腈-0.1%磷酸(20：80)；流速1.0 ml/min，柱温30℃，检测波长为322 nm。结果阿魏酸在5.18~103.6μg/ml 范围内，呈良好的线性关系(r=0.9993)，回收率为97.6%，RSD 为1.2%。结论本方法快速、准确，方法的稳定性、重复性良好，可作为℃坤宁片质量分析控制方法。 【总页数】2页(202-203)
【关键词】益坤宁片;阿魏酸;高效液相色谱法
HPLC 测定益坤宁片中阿魏酸的含量
