熊果酸对荷人骨肉瘤裸小鼠移植瘤生长的影响
陈伟;刘海生;魏萌;王丽红;师艳艳
【期刊名称】《现代肿瘤医学》 【年(卷),期】2017(025)007
【摘要】Objective:To investigate the effects of ursolic acid (UA)on xenografts growth in osteosarcoma of nude mice. Methods:Nude mice models bearing human osteosarcoma were made by injecting inoculation human osteosarco-ma cancer MG - 63 cell line and were treated with different concentrations of UA [5,10,20mg/(kg·d)]and cispl-atin (2mg/ kg)for 5 days,once a day. The histopathological changes of tumor tissue was observed by HE staining. The tumor cells apoptosis was detected by TUNEL. The expression of caspase - 3,Bax,bcl - 2 protein were detected by IHC and were semi - quantitative analyzed. Results:Compared with model group,the necrosis presentation,tumor cell shrinkage and other pathological morphological changes appeared. The number of apoptotic cells in tumor tissue of UA treated groups were significantly increased. The tumor weight were decreased and the inhibition rate were increased. The expression of Bax and caspase - 3 were significantly increased and the bcl - 2 protein was significantly de-creased,and the ratio of Bax/ bcl - 2 were significantly increased. All of the effects of UA above were dose - depend-ent with a certain. Conclusion:UA had inhibitive effects on xenografts growth in
熊果酸对荷人骨肉瘤裸小鼠移植瘤生长的影响
