HPCE法研究番茄中类胡萝卜素的动态变化
刘长付;陈媛梅;郑彩霞
【期刊名称】《中国农业大学学报》 【年(卷),期】2013(018)004
【摘要】本研究建立了高效毛细管电泳法(HPCE)测定番茄中番茄红素和β-胡萝卜素含量的分析方法.以丙酮∶石油醚为溶剂,超声提取番茄中的类胡萝卜素,经分离纯化后作供试液,确定了HPCE法检测番茄中类胡萝卜素的最佳工艺:检测波长为450 nm,运行缓冲液为pH4.5的HAc-NaAc溶液,电泳电压24 kV,0.5 MPa压力下进样6s,电泳温度为20℃.最佳工艺下,样品可在16 min内完全分离,线性关系良好.该法准确、快速、简便,可同时检测样品中的番茄红素和β-胡萝卜素.此外,按照最佳条件对5种不同成熟期的样品进行分析,发现番茄成熟过程中番茄红素的含量逐渐升高,β-胡萝卜素的含量先下降后上升,两者含量均在果实完全成熟时达到最高.%A high performance capillary electrophoresis(HPCE) method was found for measuring the content of lycopene and β-carotene in tomato.The samples were extracted with the mixed solvent of acetone and petroleum ether by ultrasonic treatment,which were used as the test solution after separation and purification.The optimal experimental
conditions,such
as
concentration
and
pH
of
buffer,separation voltage,separation temperature and injection time on the separation efficiency were determined first.The experimental conditions were as the followings:detection wave length was 450 nm,running buffer was pH 4.5,HAc-NaAc was 20 mmol/L,electrophoresis
HPCE法研究番茄中类胡萝卜素的动态变化
