利用靶向非核糖体肽合成酶的PCR技术从链霉菌HMU0027中发现thiazostatin类嗜铁素
刘发旺;周梦洁;金晶;杨小燕;张英涛;马明;杨东辉
【期刊名称】《中国药学（英文版）》 【年(卷),期】2017(026)010
【摘要】Nonribosomal peptides (NRPs) represent a large family of natural products with great diversities of chemical structures and biological activities.The peptide backbones of NRPs are synthesized by nonribosomal peptide synthetases (NRPSs) that minimally consist of one adenylation (A) domain,one peptidyl carrier protein (PCP) and one condensation (C) domain.In this study,we carried out a PCR screening and identified 21 \strains from 100 actinomycete strains with the degenerate primers designed from the conserved sequences of A domains of NRPSs.One of the 21 \strains,Streptomyces sp.HMU0027,was selected for large-scale fermentation (9 L) based on HPLC analysis,and subsequent isolation and structural elucidation afforded seven NRPS-synthesized thiazostatin siderophore analogues (1-7).Compound 1 was a new compound containing an unusual linkage between a phenolate siderophore and a sugar moiety.These results laid the foundation for further biosynthetic research of these thiazostatin analogues and highlighted the power of genome mining technologies based on biosynthetic knowledge in NRP discovery.%非核糖体肽(NRP)是
利用靶向非核糖体肽合成酶的PCR技术从链霉菌HMU0027中发现thiazostatin类嗜铁素
