γH2AX预测肺癌患者化疗敏感性的研究
张贵慧;周萍;董贺;孙青
【期刊名称】《中国肿瘤临床》 【年(卷),期】2011(038)010
【摘要】Objective: To study the feasibility and reliability of using phosphorylated histone H2AX ( γH2AX ) as a biomarker for predicting the sensitivity of a pulmonary carcinoma to cisplatin ( DDP ), carboplatin ( CBP ), gemcitabine ( GEM ), and adriamycin ( ADM ).Methods: Pulmonary carcinoma cells from the patients were primarily cultured and exposed to the above chemotherapeutic drugs at different concentration indices ( CI ).The proportion of the pulmonary carcinoma cells with positive expression of γH2AX was measured by flow cytometry and immunocytochemistry, whereas cell proliferation was measured with MTT assay.The difference between the number of γH2AX foci, the percentage of the positive cells that expressed and γH2AX, and the inhibitory rate of cell proliferation were analyzed and their correlations were compared.Results: There was correlation between the number of γH2AX foci, the percentage of positive cells expressing γH2AX, and the inhibitory rate of cell proliferation after treatment with different CI of the same drug ( all P ＜ 0.05 ).A correlation was also found among the different drugs ( all P ＜ 0.05 ).Conclusion: Using γH2AX expression for predicting the pharmacodynamic sensitivity of
γH2AX预测肺癌患者化疗敏感性的研究
