E. coli genotypes
Contents
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1 Nomenclature & Abbreviations ? 2 Methylation Issues in E. coli ? 3 Commonly used strains
o 3.1 AG1 o 3.2 AB1157 o 3.3 B2155 o 3.4 BL21 o 3.5 BL21(AI) o 3.6 BL21(DE3)
o 3.7 BL21 (DE3) pLysS o 3.8 BNN93 o 3.9 BNN97
o 3.10 BW26434, CGSC Strain # 7658 o 3.11 C600
o 3.12 C600 hflA150 (Y1073, BNN102) o 3.13 CSH50 o 3.14 D1210 o 3.15 DB3.1 o 3.16 DH1 o 3.17 DH5α
o 3.18 DH5α Turbo (NEB) o 3.19 DH10B (Invitrogen) o 3.20 DH12S (Invitrogen) o 3.21 DM1 (Invitrogen)
o 3.22 E. cloni(r) 5alpha (Lucigen) o 3.23 E. cloni(r) 10G (Lucigen) o 3.24 E. cloni(r) 10GF' (Lucigen) o 3.25 E. coli K12 ER2738 (NEB) o 3.26 ER2566 (NEB) o 3.27 ER2267 (NEB) o 3.28 HB101
o 3.29 HMS174(DE3)
o 3.30 High-Control(tm) BL21(DE3) (Lucigen) o 3.31 High-Control(tm) 10G (Lucigen) o 3.32 IJ1126 o 3.33 IJ1127
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o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o
3.34 JM83 3.35 JM101 3.36 JM103 3.37 JM105 3.38 JM106 3.39 JM107 3.40 JM108 3.41 JM109
3.42 JM109(DE3) 3.43 JM110 3.44 JM2.300 3.45 LE392 3.46 Mach1 3.47 MC1061 3.48 MC4100 3.49 MG1655 3.50 OmniMAX2
3.51 OverExpress(tm)C41(DE3) (Lucigen)
3.52 OverExpress(tm)C41(DE3)pLysS (Lucigen) 3.53 OverExpress(tm)C43(DE3) (Lucigen)
3.54 OverExpress(tm)C43(DE3)pLysS (Lucigen) 3.55 Rosetta(DE3)pLysS
3.56 Rosetta-gami(DE3)pLysS 3.57 RR1 3.58 RV308
3.59 SOLR (Stratagene) 3.60 SS320 (Lucigen) 3.61 STBL2 (Invitrogen) 3.62 STBL3 (Invitrogen) 3.63 STBL4
3.64 SURE (Stratagene) 3.65 SURE2 (Stratagene) 3.66 TG1 (Lucigen)
3.67 TOP10 (Invitrogen) 3.68 Top10F' (Invitrogen) 3.69 W3110 3.70 WM3064
3.71 XL1-Blue (Stratagene)
3.72 XL1-Blue MRF' (Stratagene) 3.73 XL2-Blue (Stratagene)
3.74 XL2-Blue MRF' (Stratagene) 3.75 XL1-Red (Stratagene) 3.76 XL10-Gold (Stratagene)
3.77 XL10-Gold KanR (Stratagene)
4 Other genotype information sources ? 5 References
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Nomenclature & Abbreviations
A listed gene name means that gene carries a loss of function mutation, a Δ preceding a gene name means the gene is deleted. If a gene is not listed, it is not known to be mutated. Prophages present in wt K-12 strains (F, λ, e14, rac) are listed only if absent. E. coli B strains are naturally lon- and dcm-.
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F- = Does not carry the F plasmid
F+ = Carries the F plasmid. The cell is able to mate with F- through conjugation.
F'[ ] = Carries an F plasmid that has host chromosomal genes on it from a previous recombination event. This cell can also mate with F- through conjugation. Chromosomal genes carried in the F plasmid are listed in brackets.
rB/K+/- = The (B/K) defines the strain lineage. The +/- indicates whether the strain has or hasn't got the restriction system.
mB/K+/- = The (B/K) defines the strain lineage. The +/- indicates whether the strain has or hasn't got the modification (methylation) system.
hsdS = Both restriction and methylation of certain sequences is deleted from the strain. If you transform DNA from such a strain into a wild type strain, it will be degraded.
hsdR = For efficient transformation of cloned unmethylated DNA from PCR amplifications
INV( ) = chromosomal inversion between locations indicated
ahpC = mutation to alkyl hydroperoxide reductase conferring disulfide reductase activity
ara-14 = cannot metabolize arabinose
araD = mutation in L-ribulose-phosphate 4-epimerase blocks arabinose metabolism
cycA = mutation in alanine transporter; cannot use alanine as a carbon source
dapD = mutation in succinyl diaminopimelate aminotransferase leads to succinate or (lysine + methionine) requirement
Δ( ) = chromosomal deletion of genes between the listed genes (may include unlisted genes!)
dam = adenine methylation at GATC sequences exist; high recombination efficiency; DNA repair turned on
E. coli 常用菌株大全
