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HIGH EFFICIENCY GENE TRANSFER AND EXPRESSION IN MAMMALIAN CELLS BY A MULTIPLE TRANSFECTION PROCEDURE OF MATRIX ATTACHMENT REGION SEQUENCES

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HIGH EFFICIENCY GENE TRANSFER AND EXPRESSION

IN MAMMALIAN CELLS BY A MULTIPLE TRANSFECTION PROCEDURE OF MATRIX ATTACHMENT REGION SEQUENCES

申请(专利)号: EP20040790766

申请日: 2004-10-22 专利号: 主分类号: 申请权利人: 公开国代码: 优先权国家: 摘 要:

EP1675952A2 C12N15/11

SELEXIS S.A.

EP EP;

US

公开公告日: 分类号: 发明设计人: 申请国代码: 优先权: 摘 要 附 图:

2006-07-05

C12N15/11;

G06F19/00; C12N5/10; A01K67/027; C12N15/67; C12N15/79 MERMOD,

NICOLAS;

GIROD, PIERRE ALAIN;

BUCHER, PHILIPP; NGUYEN, DUC-QUANG;

CALABRESE, DAVID;

SAUGY, DAMIEN; PUTTINI, STEFANIA EP

20031024 US

513574 P; 20040206 EP 04002722

The present invention relates to purified and isolated DNA sequences having protein production increasing activity and more specifically to the use of matrix

attachment regions (MARs) for increasing protein production activity in a eukaryotic cell. Also disclosed is a method for the identification of said active regions, in particular MAR nucleotide sequences, and the use of these characterized active MAR sequences in a new multiple transfection method. 主权项:

1. A purified and isolated DNA sequence having protein production increasing activity characterized

权 利 要 求 说 明 书

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HIGH EFFICIENCY GENE TRANSFER AND EXPRESSION IN MAMMALIAN CELLS BY A MULTIPLE TRANSFECTION PROCEDURE OF MATRIX ATTACHMENT REGION SEQUENCES

HIGHEFFICIENCYGENETRANSFERANDEXPRESSIONINMAMMALIANCELLSBYAMULTIPLETRANSFECTIONPROCEDUREOFMATRIXATTACHMENTREGIONSEQUENCES申请(专利)号:EP20040790766申请日:2004-10-2
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