RP-HPLC测定橘红化痰丸中苦杏仁苷的含量
蔡桂萍;司徒伟良;宋粉云;钟兆健
【期刊名称】《食品与药品》 【年(卷),期】2015(000)005
【摘要】Objective To establish a method for determination of amygdalin in Juhong Huatan Wan.MethodsA RP-HPLC method was established. The chromatographic column was Diamonsil C18. The mobile phase was a mixture of methanol-0.1 % phosphoric acid (20:80,v/v). The flow rate was 1.0 mL/min, and the detection wavelength was 210 nm. The column temperature was 30℃. ResultsThe linear range of amygdalin was 5.5-44.0μg/mL (r=0.9991). The average recovery for amygdalin was 100.3 %,withRSD of 0.84 % (n=6).Conclusion This method can be used for quantitative determination of amygdalin in the preparation.%目的建立反相高效液相色谱法（RP-HPLC）测定橘红化痰丸中苦杏仁苷含量的方法。方法色谱柱为Diamonsil C18；流动相为甲醇-0.1%磷酸水溶液（20：80），流速1.0 mL/min；检测波长210 nm；柱温30℃。结果苦杏仁苷在5.5~44.0μg/mL范围内有良好的线性关系（r=0.9991），平均回收率为100.3%，RSD为0.84%（n=6）。结论该法可用于橘红化痰丸中苦杏仁苷的含量测定。
【总页数】4页(360-363)
【关键词】橘红化痰丸;苦杏仁苷;反相高效液相色谱法;含量测定 【作者】蔡桂萍;司徒伟良;宋粉云;钟兆健
RP-HPLC测定橘红化痰丸中苦杏仁苷的含量
