CHARACTERIZATION OF LACTATE DEHYDROGENASE ISOZYME PATTERN AND MORPHOLOGY OF THREE MARINE F

CHARACTERIZATION OF LACTATE DEHYDROGENASE ISOZYME PATTERN AND MORPHOLOGY OF THREE MARINE FISH CELL LINES

郭华荣; 张士璀; 李红岩; 童裳亮; 相建海

【期刊名称】《《中国海洋湖沼学报（英文版）》》 【年(卷),期】2002(020)003

【摘要】Three continuous marine fish cell lines of FG (i.e. , Flounder Gill) from flounder (Paralichthys olivaceus) gill, SPH (i.e., Sea Perch Heart) fro m sea perch (Lateolabrax japonicus) heart and RSBF (i.e., Red Sea Bream Fin) from red se a bream (Pagrosomus major) fin, were characterized by lactate dehydrogenase (LDH) is ozyme and morphological analysis. The LDH isozyme patterns of these three cell lines and their corresponding tissues of origin were investigated and compared. The results sho wed: (1) No difference was found in the LDH isozyme patterns of FG and flounder gill tissue. However, the LDH isozyme patterns of SPH and RSBF were significantly different from their cor responding tissues of origin; (2) LDH isozyme patterns of FG, SPH and RSBF were markedly di fferent from each other and could serve as genetic markers for species identification and de tection of cross contamination. Morphological change analysis of these three cell lines in compa rison to their original tissues indicated that FG cells still appeared epithelioid without mor phological transformation. However, morphological changes were

found in SPH and RSBF compa red to their original tissues. Therefore, the cellular morphology was still plastic in the relatively stable culture conditions, and it was possible that change of LDH patterns was related to morphological changes of fish cells in vitro. 【总页数】6页(226-231)

【关键词】cell line; lactate dehydrogenase; cellular morpho logy; flounder; sea perch; red sea bream

【作者】郭华荣; 张士璀; 李红岩; 童裳亮; 相建海

【作者单位】Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China；Ocean University of Qingdao Qingdao 266003 China; Ocean University of Qingdao Qingdao 266003 China; Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China

【正文语种】中文 【中图分类】Q95 【文献来源】

https://www.zhangqiaokeyan.com/academic-journal-cn_chinese-journal-oceanic-limnology-english-edition_thesis/0201273474601.html 【相关文献】

1.CHARACTERIZATION OF LACTATE DEHYDROGENASE ISOZYME PATTERN AND MORPHOLOGY OF THREE MARINE FISH CELL LINES [J], 郭华荣; 张士璀; 李红岩; 童裳亮; 相建海

2.Establishment and Characterization of a New Marine Fish Cell Line from Ovary of Barfin Flounder (Verasper moseri) [J], XU Xiaohui; FAN Tingjun; JIANG Guojian; YANG Xiuxia

3.Potential of four marine-derived fungi extracts as anti-proliferative and cell death-inducing agents in seven human cancer cell lines [J], Alice Abreu Ramos; Maria Prata-Sena; Bruno Castro-Carvalho; Tida Dethoup; Suradet Buttachon; Anake Kijjoa; Eduardo Rocha

4.Consumer Preference and Consumption Pattern of Marine Fish Species in Ibadan Metropolis, Oyo State, Nigeria [J], Tosan B. Fregene; Adeniyi S. Olanusi

5.Isolation and characterization of cancer stem-like cells from MHCC97H Cell Lines [J], Shanyong Yi; Kejun Nan; Aihua Yuan; Chuangxin Lu

以上内容为文献基本信息，获取文献全文请下载