线粒体复合物及 ATP 酶活性在不同月龄帕金森病模型小鼠
的差异
马琳;牛海玲;郭记宏;付廷飞
【期刊名称】《脑与神经疾病杂志》 【年(卷),期】2016(024)007
【摘要】Objective To observe the the differences of mitochondrial respiratory function in mice with different age of Parkinson's disease (PD). Method C57BL/6 female mice of 8-month and 16-month were divided into 4 groups in random: 8-month control group, 8-month model group, 16-month control group and 16-month model group. 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP) was injected subcutaneously for two model groups to make Parkinson’s disease model, while the same volume of normal saline was injected to control groups. Examined their behavioral changes and detected contacts of dopamine in their nigra. The activity of complex Ⅰ - Ⅳ were tested by the ultraviolet spectroscopy, while the synthetic activity of ATP was measured by luciferase chemiluminescence detection. Results The behavior performance were abnormal and dopamine contacts, complex Ⅰ activity and ATP synthesis activity were all decreased in model groups compared with control groups (P<0.05). Moreover, all the scores were poorer in 16-month mice than in 8-month model mice (P<0.05). The differences among these groups for the activity of complex Ⅱ , Ⅲ , Ⅳ
线粒体复合物及 ATP 酶活性在不同月龄帕金森病模型小鼠的差异
