Antimicrobial activity of alexidine alone and associated with N-acetylcysteine against Ent

Antimicrobial activity of alexidine alone and associated with N-acetylcysteine against

Enterococcus faecalis biofilm

Luiz Fernando Machado Silveira;Pilar Baca;Mara Teresa Arias-Moliz;Alberto Rodrguez-Archilla;Carmen Mara Ferrer-Luque

【期刊名称】《国际口腔科学杂志（英文版）》 【年(卷),期】2013(000)003

【摘要】The purpose of this study was to assess the efficacy of alexidine (ALX), alone and combined with N-acetylcysteine (NAC), in eradicating two Enterococcus faecalis strain biofilms. The biofilms of E. faecalis ATCC 29212 and the clinical isolate E. faecalis D1 were grown in the MBEC-high-throughput device for 24 h and were exposed to five twofold dilutions of ALX (2%-0.007 8%) alone and combined with 100 mg?mL21 NAC, for 1 and 5 min. Eradication was defined as 100%kill of biofilm bacteria. The Student’s t-test was used to compare the efficacy of the associations of the two irrigants. After 1-min contact time, ALX eradicated the biofilms at all concentrations except for 0.007 8%and 0.015 6%-0.007 8%with E. faecalis ATCC 29212 and E. faecalis D1, respectively. Similar results for eradication and concentration were obtained when it was combined with 100 mg?mL21 NAC. After 5 min of contact time, ALX alone and combined with NAC eradicated all enterococci biofilms. ALX showed antimicrobial properties against the