高蛋白、低碳水化合物饮食对营养性肥胖大鼠体重和胃肠
激素表达的影响
陈海燕;马立川;李茵茵;赵家军;李明龙
【期刊名称】《中华预防医学杂志》 【年(卷),期】2011(045)002
【摘要】目的 观察长期高蛋白、低碳水化合物饮食对营养性肥胖大鼠体重和胃肠激素表达的影响. 方法 利用高脂饲料建立营养性肥胖大鼠模型24只,用随机数字表法按体重分层随机分为高蛋白饲养组(HP组,36.7%蛋白质)、普通饲养组(NC组,22.4%蛋白质),每组12只.正常等热量饲养24周后,测定体重、内脏脂肪及空腹血浆胃促生长素、胰高血糖素样肽1(GLP-1),免疫组织化学检测胃底胃促生长素、回肠GLP-1蛋白表达水平. 结果 24周后,NC、HP组体重分别为(490.92±39.47)、(545.55±31.08)g(t=-3.664,P＜0.01);内脏脂肪分别为(22.42±7.04)、(32.33±9.27)g(t=-2.503,P＜0.05);空腹血浆胃促生长素分别为(2.36±0.82)、(1.95±0.64)ng/ml(t=1.337,P＞0.05),并与体重(r=-0.370,t=-1.899,P＜0.05)、内脏脂肪量(r=-0.454,t=-2.52,P＜0.01)呈负相关;空腹血浆GLP-1分别为(0.52±0.13)、(0.71±0.19)μg/L(t=-2.758,P＜0.05);胃底胃促生长素表达分别为25 473±8701、10 526±6194(t=2.501,P＜0.05);回肠GLP-1蛋白表达分别为27 431±5813、36 601±5083(t=-1.833,P=0.081). 结论 长期高蛋白、低碳水化合物饮食可引起营养性肥胖大鼠体重、体脂下降,胃促生长素表达升高,GLP-1下降.%Objective To investigate the effects of long-term high-protein,low-carbohydrate diet on body weight and the expression of gastrointestinal hormones in diet-induced obesity rats.
Methods Twentyfour diet-induced obesity rat models were established by feeding fat-enriched diet,then were randomly divided into two groups by stratified sampling method by weight:the high-protein diet group (HP,36.7% of energy from protein),and the normal chow group (NC,22.4% of energy from protein),12 rats in each group.The total calorie intake of each rat per day was similar and was maintained for 24 weeks,then body weight,visceral fat mass,fasting plasma ghrelin and glucagon-like peptide-1 (GLP-1) were determined,as well as protein expression of ghrelin in stomach,GLP-1 in ileum were detected by immunohistochemistry. Results After 24 weeks,body weight of HP,NC groups were (490.92±39.47)g and (545.55±31.08)g,respectively (t=-3.664,P＜0.01);visceral fat mass were (22.42±7.04)g and (32.33±9.27) g,respectively(t=-2.503,P＜0.05);plasma ghrelin level were (2.36±0.82) and (1.95±0.64) ng/ml,respectively(t=1.337,P＞0.05),and plasma ghrelin level was negatively correlated to body weight (r=-0.370,t=-1.899,P＜0.05),visceral
fat
mass(r=-0.454,t=-2.52,P＜0.01);plasma
GLP-1
concentration were (0.52±0.13)and(0.71±0.19)ng/ml,respectively(t=-2.758,P＜0.05);ghrelin protein expression in stomach were 25 473±8701 and 10 526 ± 6194,respectively (t=2.501,P＜0.05);GLP-1 prot ein expression in ileum were 27 431±5813 and 36 601±5083,respectively (t=-1.833,P=0.081). Conclusion Long-term isocaloric high-protein,low-carbohydrate diet can reduce body weight and visceral fat,increase the
expression of ghrelin,and decline GLP-1 expression in diet-induced obesity rats.
【总页数】4页(132-135)
【关键词】肥胖症;膳食;蛋白质类;碳水化合物;体重减轻;胃肠激素类 【作者】陈海燕;马立川;李茵茵;赵家军;李明龙
【作者单位】250021,济南,山东大学附属省立医院保健内分泌科;河北大学附属医院内分泌科;250021,济南,山东大学附属省立医院保健内分泌科;250021,济南,山东大学附属省立医院保健内分泌科;250021,济南,山东大学附属省立医院保健内分泌科
【正文语种】中文 【中图分类】 【文献来源】
https://www.zhangqiaokeyan.com/academic-journal-cn_chinese-journal-preventive-medicine_thesis/0201215363285.html 【相关文献】
1.以低碳水化合物饮食模式为主的体重管理对超重及肥胖人群的干预研究 [J], 施万英; 吕婵; 张译文; 吕青青; 朱华倩; 陆金鑫
2.低碳水化合物高蛋白质膳食对肥胖大鼠影响的研究 [J], 杨月欣; 刘兰; 王竹 3.碳水化合物——无力：缺乏碳水化合物肌肉疲乏无力吗？ [J],
4.康乃尔净碳水化合物蛋白质系统评价常用饲料碳水化合物和蛋白质瘤胃降解 [J], 薛红枫; 任丽萍; 周振明; 孟庆翔
5.肥育后期喂高蛋白/低碳水化合物日粮对猪肉品质的影响 [J], J．M．Leheska;
高蛋白、低碳水化合物饮食对营养性肥胖大鼠体重和胃肠激素表达的影响
