TNP-470、顺铂联合应用抗人舌癌生长的实验研究
邓永强;邱蔚六;陈万涛;李为国
【期刊名称】《现代口腔医学杂志》 【年(卷),期】2001(015)002
【摘要】目的 研究血管生成抑制剂TNP-470对人舌癌生长的抑制作用。方法 MTT法测定TNP-470、顺铂(CDDP)对体外培养人舌癌Tca8113细胞增殖的抑制作用。用Tca8113细胞形成裸鼠肿瘤皮下移植模型，移植二 周后腹腔注射用药，TNP-470(30mg/kg)隔日一次，共七次，CDDP(5mg/kg)一周二次，共四次，同期测量肿瘤体积和裸鼠体笪”移植四周后处死动物，检测肿瘤体积和主要脏器。结果 TNP-470作用Tca8113细胞的半数抑制浓度(IC50)为70.79 μg/ml。TNP-470组、CDDP组和TNP-470+CDDP组抑瘤率分别为31.7%、40.3%和65. 6%，实验组与对照组之间抑瘤率有显著性差异(P＜0.05)，TNP-470+CDDP组抑瘤效果最佳。结论 TNP-470对人舌癌体内生长有一定抑制作用，联合应用CDDP可进一步提高治疗效果。%Objective．To investigate the effect of the angiogenesis inhibitor TNP-470 on the growth of human tongue cancer.Methods．The cytotoxicity of TNP-470、CDDP on human tongue cancer cells (Tca8113) was detected with MTT assay in vitro.A human tongue cancer model was builtby tumor bearing mude mice using Tca8113.TNP-470(30mg/kg) and CDDP(5m g/kg) were administrated I.p.after two weeks of implantation.Tumor volumes and a nimal weights were measured simultaneously.Autopsy was performed after four weeks of implantation.Results．The 50% inhibitory
TNP-470、顺铂联合应用抗人舌癌生长的实验研究
