芍药苷对脂肪组织释放游离脂肪酸及抗氧化作用的影响
刘雨娟;黄征;蔺美玲;张小都;郑天珍
【期刊名称】《中国老年学杂志》 【年(卷),期】2011(031)017
【摘要】目的 观察芍药苷对大鼠离体脂肪组织释放游离脂肪酸(FFA)及抗氧化作用的影响,并初步探讨其作用机制.方法 取健康成年SD大鼠腹膜后脂肪组织,放入37℃的含有小牛血清白蛋白(0.04 g/ml)的Krebs液中温育90 min,测定FFA、丙二醛( MDA)和总抗氧化能力(T-AOC).结果 芍药苷可促进离体脂肪组织释放FFA,同时降低MDA的含量,提高T-AOC水平,且对FFA的影响作用存在相应的剂量效应关系.普萘洛尔、维拉帕米,腺苷酸环化酶(AC)抑制剂( SQ22,536)可不同程度地阻断芍药苷促进离体脂肪组织释放FFA及降低MDA和提高T-AOC的作用.结论 芍药苷促进离体脂肪组织释放FFA及降低MDA的含量,提高T-AOC水平的作用机制可能经肾上腺素能β受体、维拉帕米敏感的L型Ca2+通道及cAMP作为第二信使介导的信号转导途径.%Objective To investigate the effects of paeoniflorin on releasing of free fatty acids ( FFA)and anti-oxidative capacity in isolated lipid tissue in rats and explore its preliminary mechanism. Methods Some retroperitoneal adipose tissue of healthy adult SD rats were selected and taken away. They were incubated into Krebs solution that it contains BSA (0.4% ) for 90 min. Then some solution was draw to check up the quantity contained of FFA, malondialdehyde ( MDA), total anti-oxidative capacity (T-AOC ). Results Paeoniflorin could promote the release of FFA from the adipose tissue,
芍药苷对脂肪组织释放游离脂肪酸及抗氧化作用的影响
