胰腺癌早期诊断相关miRNAs的筛选与验证
夏蜀珺;郭俊超;李建;周立;赵玉沛
【期刊名称】《中华外科杂志》 【年(卷),期】2014(052)003
【摘要】Objective To find the potential serum specific miRNAs with diagnostic value in early pancreatic cancer and study the alteration of miRNAs levels in the process of origin and development of pancreatic cancer and discuss the diagnostic value of miRNAs in early pancreatic cancer.Methods DMBA-induced rat model was established.The miRNAs expression profile of early stage was screened out by microarray.And confirmation study was performed.Results The 35 and 12 abnormally expressed miRNAs were acquired in pancreatic tissue and blood respectively.There were no significant differences between normal pancreas and pancreatic cancer in expressions of hsa-let-7c,hsa-miR-122-5p,hsa-miR-142-5p,hsamiR-199a-3p and hsa-miR-451 a (P ＞0.05).Conclusions miRNAs are the potential biomarkers of early pancreatic cancer.The establishment of the miRNAs expression profile has build the foundation of exploring the molecular mechanism of origin of pancreatic cancer.%目的 寻找具有潜在早期诊断价值的外周血特异性miRNAs；探索胰腺癌发生过程中相关miRNAs水平的动态变化及其对胰腺癌早期诊断的价值.方法 通过建立SD大鼠胰腺癌演进模型,利用miRNA芯片技术筛选胰腺癌发生相关的差异miRNA表达谱,并利用生物信息学技术对相关
胰腺癌早期诊断相关miRNAs的筛选与验证
