Cloning and bioinformatical analysis of the N-terminus of the sonic hedgehog gene

Cloning and bioinformatical analysis of the N-terminus of the sonic hedgehog gene

Yi Zhang;Shu Zhao;Weiren Dong;Suifen He;Haihong Wang;Lihua Zhang;Yinjuan Tang;Jiasong Guo;Suiqun Guo

【期刊名称】《中国神经再生研究（英文版）》 【年(卷),期】2013(008)003

【摘要】The sonic hedgehog protein not only plays a key role in early embryonic development, but also has essential effects on the adult nervous system, including neural stem cell proliferation, differentiation, migration and neuronal axon guidance. The N-terminal fragment of sonic hedgehog is the key functional element in this process. Therefore, this study aimed to clone and analyze the N-terminal fragment of the sonic hedgehog gene. Total RNA was extracted from the notochord of a Sprague-Dawley rat at embryonic day 9 and the N-terminal fragment of sonic hedgehog was amplified by nested reverse transcription-PCR. The N-terminal fragment of the sonic hedgehog gene was successfully cloned. The secondary and tertiary structures of the N-terminal fragment of the sonic hedgehog protein were predicted using Jpred and Phyre online. 【总页数】6页(258-263) 【关键词】

【作者】Yi Zhang;Shu Zhao;Weiren Dong;Suifen He;Haihong