糖原合成酶激酶3β对卵巢癌紫杉醇耐药性的影响及机制研
究
朱饮源;姚军;李曼
【期刊名称】《东南国防医药》 【年(卷),期】2015(000)001
【摘要】Objective To study the effects of GSK-3βon paclitaxel chemoresistance of ovarian cancer cells .Methods SK-OV3 cells was treated with LiCl 20 mmol/L for 1 hour to enhance the expression of p-GSK-3β(ser9) which is inactivated.MTT was used to test the IC50 of paclitaxel.The expression of p-GSK-3β(ser9) was tested by FACS.The location of p-GSK-3β(ser9) in SK-OV3 cells was analyzed by immunofluorescence .Results After treated with LiCl, the IC50 of paclitaxel was increased from (10.11 ± 0.87) μg/mL to (11.56 ±0.96) μg/mL for 24 h, (1.07 ±0.17) μg/mL to (1.74 ±0.24) μg/mL for 48 h, (0.03 ±0.01)μg/mL to (0.06 ±0.01) μg/mL for 72 h respectively.Except the increase of 24 h others had statistical difference (P<0.05).FACS results show that the mean fluorescence intensity of p-GSK-3βincreased from (661.67 ±37.63) to (841.67 ±41.53) after treated with LiCl(P<0.05).The results of immunofluorescence show that the expression of p-GSK-3βin nuclear increased obviously than in the cytoplasm.Conclusion The augment of inactivated GSK-3βexpression, especially in the nuclear, can improve the chemoresis-tance of ovarian cancer cells .%目的：探讨糖原
糖原合成酶激酶3β对卵巢癌紫杉醇耐药性的影响及机制研究
