Intratumoral Expression of MIP-1β Induces Antitumor Responses in a Pre-Established Tumor

Intratumoral Expression of MIP-1β Induces Antitumor Responses in a Pre-Established Tumor Model through Chemoattracting T Cells and NK

Cells

XiaolingLuo;YizhiYu;AnminLiang;YuanXie;ShuxunLiu;JunGuo;WenyaWang;RunziQi;HuazhangAn;MinghuiZhang;HongmeiXu;ZhenhongGuo;XuetaoCao

【期刊名称】《细胞与分子免疫学：英文版》 【年(卷),期】2004(001)003

【摘要】Direct intratumoral introduction of therapeutic or regulatory genes is a developing technology with potential application for cancer gene therapy. Macrophage inflammatory protein-1 beta (MIP-1β) is a chemokine which can chemoattract immune cells such as T cells. In the present study, murine colorectal adenocarcinoma CT26 cells were transfected with a recombinant adenovirus (AdhMIP-1β) carrying the human MIP-1β gene. 24h post-transfection, hMIP-1β levels reached approximately 980 pg/ml in supernatants of 106 hMIP-1β-transfected CT26 cells. Moreover, the supernatants exhibited chemotactic activity for CD8+ T cells, CD4+ T cells, NK cells and immature DCs. Intratumoral injection of AdhMIP-1β significantly inhibited tumor growth and prolonged the survival time of tumor-bearing mice. Intratumoral hMIP-1β gene transfer also induced powerful tumor-specific CTL responses in vivo. The therapeutic effects of hMIP-1β gene therapy were greatly