微小RNA-155对胃癌细胞株SGC7901生物学行为的影
响
宋军;樊瑞智;徐溢新;宋虎;付海啸;白津;徐为
【期刊名称】《中华实验外科杂志》 【年(卷),期】2015(032)011
【摘要】目的 观察微小RNA-155(miR-155)对胃癌细胞增殖、凋亡、侵袭及迁移的影响.方法 实验分未转染组、空白对照组、miR-155模拟物转染组及miR-155抑制物转染组4组,分别将miR-155模拟物及抑制物瞬时转染胃癌细胞株SGC7901,实时定量反转录聚合酶链反应(RT-qPCR)检测转染细胞的miR-155表达水平,细胞计数试剂盒(CCK-8法)检测细胞增殖能力,流式细胞术检测细胞凋亡及细胞周期,Transwell小室检测细胞体外侵袭迁移能力.结果 RT-qPCR显示:转染miR-155模拟物后,SGC7901细胞miR-155表达水平明显上调,而转染miR-155抑制物后表达明显受抑(P＜0.01).miR-155模拟物转染组细胞增殖活性、迁移和侵袭能力明显增强(P＜0.05),凋亡率(1.68±0.34)％较未转染组(4.23±0.52)％、空白对照组(3.43±0.61)％和抑制物转染组(9.10±2.13)％明显降低(P＜0.05).转染miR-155抑制物后,出现G0/G1期阻滞,G0/G1期细胞增多达(70.63±1.12)％.结论 miR-155能促进胃癌细胞株SGC7901增殖,抑制其凋亡,增强其迁移及侵袭能力.%Objective To explore the effect of microRNA-155 (miR-155) on the biological behaviors of human gastric carcinoma cells.Methods Experiments were divided into four groups : negative control group and blank control group, miR-155 mimics group, and miR-155 inhibitor group.miR-155 mimics group was transfected with
miR-155 mimics to enhance the functions of miR-155, and miR-155 inhibitor group was transfected with miR-155 inhibitor.miR-155 mRNA expression was detected by real-time quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR).Cell proliferation was evaluated using the cell counting kit-8 (CCK-8) assay and apoptosis was assessed by flow cytometry.Invasion and migration were measured by Transwell chamber assays.Results The SGC7901 cells transfected with miR-155 mimics showed significantly higher miR-155 mRNA expression than the non-transfected SGC7901 cells and the transfected control cells (P ＜ 0.01).The miR-155 mRNA expression was significantly lower in the SGC7901 cells transfected with the miR-155 inhibitor than the non-transfected SGC7901 cells and the transfected control cells (P ＜ 0.01).The overexpression of miR-155 promoted the viability, and invasion and migration abilities, as shown in the cells transfected with the miR-155 mimics (P ＜ 0.05).There was no significant difference between control group and blank group, while the apoptosis rate in the mimics group [(1.68 ± 0.34) ％] was lower than that in the control group [(4.23 ± 0.52) ％], blank group [(3.43 ± 0.61) ％] and inhibitor group [(9.10-± 2.13)％] (P＜0.05), and the cell cycle was significantly arrested in G0/G1 phase and the number of G0/G1 phase cells was increased larger than (70.63 ± 1.12) ％ by flow cytometry.Conclusion miR-155 had a promotable effect on cell proliferation, inhibited apoptosis, and
markedly promoted invasion and migration of SGC7901 cells. 【总页数】3页(2702-2704)
【关键词】胃癌;微小RNA-155;增殖;侵袭
【作者】宋军;樊瑞智;徐溢新;宋虎;付海啸;白津;徐为
【作者单位】221002 江苏,徐州医学院附属医院胃肠外科;221002 江苏,徐州医学院附属医院胃肠外科;221002 江苏,徐州医学院附属医院胃肠外科;221002 江苏,徐州医学院附属医院胃肠外科;221002 江苏,徐州医学院附属医院胃肠外科;江苏省肿瘤生物治疗重点实验室;221002 江苏,徐州医学院附属医院胃肠外科 【正文语种】中文 【中图分类】 【文献来源】
https://www.zhangqiaokeyan.com/academic-journal-cn_chinese-journal-experimental-surgery_thesis/0201234753809.html 【相关文献】
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微小RNA-155对胃癌细胞株SGC7901生物学行为的影响
