Conformational Changes in HL60 Cells during Differentiation and Apoptosis Induced by Genistein

Conformational Changes in HL60 Cells during Differentiation and Apoptosis Induced by Genistein

王钊;周江兵;孙素琴;刘明杰;张洪钧

【期刊名称】《清华大学学报（英文版）》 【年(卷),期】2001(006)003

【摘要】Genistein can induce not only differentiation but also apoptosis in HL60 human leukemia cells. During the differentiation and apoptosis, the HL60 cells undergo some conformational changes. Fourier transform infrared (FT-IR) was employed to detect those changes. The results

showed

that

the

contents

of

DNA/protein

and

glycoprotein/protein increased. The α-helix of the membrane protein also increased. In addition, the C-O (H) stretching mode of serine, threonine and tyrosine residues of the cell proteins also changed. Those conformational changes suggest some mechanisms for how genistein affects the HL60 cells. 【总页数】5页(243-247) 【关键词】

【作者】王钊;周江兵;孙素琴;刘明杰;张洪钧

【作者单位】Department of Biological Sciences and Biotechnology, Tsinghua

University,;Department

of

Biological

Sciences

and

Biotechnology, Tsinghua University,;Department of Chemistry, Tsinghua University,;Department of Biological Sciences and Biotechnology,