系统性红斑狼疮患者外周血中趋化因子基质细胞衍生因子-1α及其受体CXC趋化因子受体4的检测及其临床意义
祖蓓蓓;刘琳;饶咏梅;李美荣;张清林
【期刊名称】《中华风湿病学杂志》 【年(卷),期】2013(017)012
【摘要】Objective To explore the expressions of stromal cell derived factor 1 α (SDF-1α) and cysteine-X-cysteine chemokine receptor 4 (CXCR4) in the peripheral blood of patients with systemic lupus erythematosus (SLE).Methods Forty hospitalized SLE patients were recruited and twenty healthy volunteers were enrolled as normal controls.The percentage of CD3+CD4+CXCR4+,CD3+CD8+CXCR4+ and the plasma concentration level of SDF-1α in the control group and SLE patients were detected by flow cytometry and ELISA.The relationship between SDF-1α/CXCR4 and SLEDAI was explored.Kruskal-Wallis H,Pearson's and Mann-Whitney U test were used for statistical analysis.Results the expression of SDF-1α [329 (127,539) pg/ml] and CXCR4
[CD3+CD4+CXCR4+:4.20(2.01,6.35)％,CD3+CD4+CXCR4+:2.70(1.68,4.20)％] were significantly elevated in SLE patients when compared to the normal controls (Z=-6.277,-5.707,-4.885,respectively,all P=0.000),and were significantly increased in highly active SLE patients than less active SLE (Z=-5.414,-5.256,-5.312,P＜0.01).The expression of SDF-1α and
CXCR4 in the butterfly erythema group,anemia group and proteinuria group were significantly higher than the normal group (P＜0.05).Both SDF-1αand
CXCR4
were
positively
correlated
with
SLEDAI
(r=0.857,0.830,0.861,respectively,all P＜0.01).Conclusion The expressions of SDF-1α and CXCR4 increase significantly in the peripheral blood of SLE patients and there is close relationship between SDF-1α/CXCR4 and disease activity,organ damage.The results of this study suggestthat SDF-1α/CXCR4 may play an important role in the pathogenesis of SLE.%目的 初步探讨趋化因子基质细胞衍生因子-1α(SDF-1α)及其受体CXC趋化因子受体4(CXCR4)在系统性红斑狼疮(SLE)患者外周血中的表达水平及意义.方法 用流式细胞术分别检测健康对照组、SLE患者各组别中外周血CD3+CD4+T细胞、CD3+CD8+T细胞表面CXCR4表达百分率,采用酶联免疫吸附试验(ELISA)检测以上各组血清中SDF-1α浓度水平.进一步分析SDF-1α,CXCR4表达水平与SLE疾病活动指数(SLEDAI)的相关性.采用Pearson相关分析、非参数Mann-Whitney U检验、Kruskal-Wallis H检验进行统计分析.结果 SLE患者组中外周血CD3+CD4+T细胞[4.20(2.01,635)％]、CD3 +CD8+T细胞[2.70(1.68,4.20)％]表面CXCR4表达百分率及血清中SDF-1α浓度水平[329(127,539) pg/ml]均明显高于健康对照组(Z值分别为-6.277、-5.707、--4.885,P均＜0.01),SLE病情活动组患者外周血中CD3+CD4+T细胞、CD3+CD8+T细胞表面CXCR4表达百分率及血清中SDF-1α浓度水平均明显高于SLE非活动组患者(Z值分别为-5.414、-5.256、-5.312,P均＜0.01).SLE蝶形红斑患者、贫血患者、蛋白尿患者的外周血中CXCR4、SDF-1α表达水平
均较阴性组高(P均＜0.05),同时SDF-1α,CXCR4表达与SLE疾病活动指标呈正相关关系(r值分别为0.857、0.830、0.861,P均＜0.01).结论 SLE患者病情活动、组织脏器损害与SDF-1 α/CXCR4高表达有关,提示SDF-1 α/CXCR4可能在SLE的发病过程中起重要作用. 【总页数】5页(828-832)
【关键词】红斑狼疮,系统性;基质细胞衍生因子-1;CXC趋化因子受体4 【作者】祖蓓蓓;刘琳;饶咏梅;李美荣;张清林
【作者单位】221009 江苏省徐州市中心医院风湿免疫科;221009 江苏省徐州市中心医院风湿免疫科;221009 江苏省徐州市中心医院风湿免疫科;221009 江苏省徐州市中心医院风湿免疫科;221009 江苏省徐州市中心医院风湿免疫科 【正文语种】中文 【中图分类】 【文献来源】
https://www.zhangqiaokeyan.com/academic-journal-cn_chinese-journal-rheumatology_thesis/0201232355793.html 【相关文献】
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系统性红斑狼疮患者外周血中趋化因子基质细胞衍生因子-1α及其受
