2种蜜蜂和4种胡蜂蜂毒明肽基因比较
张素方;施婉君;程家安;张传溪
【期刊名称】《中国昆虫科学：英文版》 【年(卷),期】2003(010)001
【摘要】Preproapamin genes were amplified by RT-PCR from total RNA from the venom glands of 2 honeybee species, Apis mellifera, A. cerana cerana, and 4 wasp species, Vespa magnifica, V. velutina nigrothorax and Polistes hebraeus, respectively. Their PCR products were ligated into pGEM(R) -T easy vector and the nucleotide sequences analyzed. The six fragments were all 141 bp in length and contained an ORF coding the precursor of apamin.The apamin precursors of V. magnifica, V. velutina nigrothorax and P. hebraeus had 95 % and 93 % similarity with that of A. melliera in nucleotide and amino acid sequences, respectively. That of Vespula maculifrons was identical to that of A. mellifera in nucleotide and amino acid sequences. Apamin precursors of V. magnifica, P. hebraeus and V. velutina nigrothorax also had the same nucleotide sequences. The nucleotide sequences of preproapamin genes from the Chinese honeybee, A. cerana cerana and 4 wasp species are described for the first time. A notable discovery was that the wasps species had exactly same apamins as the honeybees despite the fact they belong to different insect families.%从中华蜜蜂、意大利蜜蜂和大胡蜂、墨胸胡蜂、额斑黄胡蜂、亚非马蜂6种蜂的雌成蜂毒腺中快速抽提总RNA,用RT-PCR方法
2种蜜蜂和4种胡蜂蜂毒明肽基因比较
