山定子种质资源的试管苗保存
胡月华;王忆;许雪峰;韩振海
【期刊名称】《安徽农业科学》 【年(卷),期】2009(037)031
【摘要】[ Objective ] This study aimed to establish in vitro conservation system for fruit tree Malus baccata. [ Method ] With the north common fruit tree Mains baccata as the tested materials, they were cultured on the MS basic medium added by mannitol with different concn. at 0, 5, 10, 15, 20, 25 g/L for in vitro conservation at normal temperature and low temperature(5℃)resp. to study the effect of mannitol concn. and the temperature condition on the conservation of test tube seedlings of M. baccata. [Result] Under normal temperature, adding the mannitol at 15 g/L into the MS medium could get better conservation and the test tube seedlings could be conserved for 9 months, with the conservation time being close to CK that had the conservation for 8 months. Under low temperature, the conservation cycle of the test tube seedlings was increased and adding the mannitol at 10 and 15 g/L into the MS medium could get better conservation, with the conservation cycle being delayed to 20 months. The observation on the test tube seedlings which were conserved for 16 months under normal temperature and low temperature and then were made for subculture and rooting culture, it was found that their biological characteristics were no obvious change.
[Conclusion] The optimum mannitol concn. for M. baccata concentration was 15 g/L at the normal temperature and that was 10 - 15 g/L at the low temperature.%[目的]建立果树砧木山定子离体保存体系.[方法]以北方常用果树砧木山定子的组培苗为试材,置于添加不同浓度甘露醇(0、5、10、15、20、25 g/L)的MS基本培养基上,分别在常温和低温(5 ℃)条件下进行保存,研究甘露醇浓度和温度条件对山定子试管苗保存的影响.[结果]常温下,基本培养基中添加甘露醇15 g/L的保存效果较好,试管苗可保存9个月,与对照保存8个月的效果相近;低温条件下,试管苗的保存周期明显增长,在培养基中添加10、15 g/L甘露醇的保存效果较好,保存周期延长到20个月.常温和低温下保存16个月的试管苗,经继代培养和生根培养后观察,发现其生物学性状无明显变化.[结论]常温下山定子保存最适宜的甘露醇浓度是15 g/L;低温下最适宜的甘露醇浓度是10～15 g/L. 【总页数】2页(15597,15619)
【关键词】山定子;甘露醇;种质资源;离体保存 【作者】胡月华;王忆;许雪峰;韩振海
【作者单位】中国农业大学园艺植物研究所,北京市果树逆境生理与分子生物学重点实验室,北京,100193;商丘职业技术学院,河南商丘,476000;中国农业大学园艺植物研究所,北京市果树逆境生理与分子生物学重点实验室,北京,100193;中国农业大学园艺植物研究所,北京市果树逆境生理与分子生物学重点实验室,北京,100193;中国农业大学园艺植物研究所,北京市果树逆境生理与分子生物学重点实验室,北京,100193 【正文语种】中文
【中图分类】S667.9 【文献来源】
https://www.zhangqiaokeyan.com/academic-journal-cn_journal-anhui-agricultural-sciences_thesis/020122177376.html 【相关文献】
1.茶种质资源室内保存技术研究:茶树茎切段试管苗保存技术 [J], 王立 2.甘露醇保存甘薯种质试管苗的效果 [J], 黄萍; 马朝宏; 颜谦
3.高山红景天(Rhodiola sachalinensis)试管苗缓慢生长法保存及试管苗DNA含量分析 [J], 刘剑锋; 阎秀峰; 程云清; 周晓梅; 张晓艳 4.园艺植物种质资源试管保存与鉴定 [J], 汤青林; 宋明
5.云南药用植物种质资源的试管保存技术 [J], 郑丽屏; 王玲; 李勇军
以上内容为文献基本信息，获取文献全文请下载
山定子种质资源的试管苗保存
