The Inhibitory Role of Lactacystin and β-Lactacystinon T-cell Activation and Proliferation

The Inhibitory Role of Lactacystin and β-Lactacystinon T-cell Activation and Proliferation

Peng-HongSONG; Hai-YangXIE; Shu-SenZHENG; JianWU

【期刊名称】《《生物化学与生物物理学报：英文版》》 【年(卷),期】2004(036)002

【摘要】To evaluate the effects of proteasome inhibitors lactacystin (LAC) and β-1actacystin (β-LAC)on the proliferation and activation of T lymphocytes, flow cytometry was used to analyze the proliferationand the expression of CD69, CD25 and CD3 of T lymphocytes activated by PHA. Furthermore, theexpressions of PA28 and IL-2 mRNA were assayed by competitive RT-PCR. The results indicated that:(1) LAC and 13-LAC significantly decreased the incomoration of BrdU and inhibited T lymohocytesoroliferation in T lymphocytes activated by PHA; (2) although LAC and β-LAC did not affect the expressionof CD69 at any time, they significantly inhibited the expression of CD25 (48 h, 72 h, P<0.05);(3) in comoarison with control, LAC and β-LAC significantly down-regulated the expression of PA28and 1L-2 mRNA (48 h, 72 h, P<0.05). LAC and β-LAC significantly inhibited the proliferation and activationof T cells. Mechanisms involved are inhibition of CD25 and down-regulation of PA28 and IL-2 mRNAexpressions. 【总页数】5页(P.123-127)

【关键词】T细胞; 活化; 增殖; LAC; 淋巴细胞; 流式血细胞记数法; 免疫抑制; 蛋

白质水解酶

【作者】Peng-HongSONG; Hai-YangXIE; Shu-SenZHENG; JianWU 【

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者

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位

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TheFirstAffiliatedHospital

SchoolofMedicine

ZhejiangUniversity Hangzhou310003China 【正文语种】中文 【中图分类】R392.12 【文献来源】

https://www.zhangqiaokeyan.com/academic-journal-cn_acta-biochimica-biophysica-sinica_thesis/0201274928084.html 【相关文献】

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