多囊卵巢综合征大鼠模型脂肪组织雄激素转换酶的表达研
究
王丽华;李圣贤;岳江;格日乐;韩婷婷;姚菊芳;郑璐莹;刘伟
【期刊名称】《中华内分泌代谢杂志》 【年(卷),期】2013(029)003
【摘要】目的 建立多囊卵巢综合征(PCOS)大鼠模型,了解其脂肪组织雄激素转换酶的表达变化.方法 21日龄SD大鼠分为A组(对照组:普食+油剂注射)和B组(造模组:高脂饮食+脱氢表雄酮注射),饲养9周,观察大鼠代谢及卵巢形态等变化,并比较两组大鼠脂肪组织雄激素转换酶表达差异.结果 与A组比较,B组大鼠空腹血糖、胰岛素、稳态模型评估的胰岛素抵抗指数、血清睾酮升高(P＜0.05),失去规律动情周期,卵巢呈多囊样改变；皮下、宫旁脂肪组织3β羟类固醇脱氢酶(3βHSD)表达均增加,17β羟类固醇脱氢酶l(17βHSDl)表达均下降,宫旁脂肪5α还原酶l表达亦下降(P＜0.05).结论 建立了合并胰岛素抵抗和代谢紊乱的大鼠多囊卵巢模型,其脂肪组织雄激素转换酶的表达随体内激素水平变化发生了改变.%Objective To establish a polycystic ovary syndrome (PCOS) rat model and compare the expression of testosterone converting enzymes in adipose tissue of PCOS rat with that of controls.Methods 21-dayold female SD rats were divided into two groups:group A(control group):feed pellets and injected with oil; group B (model group):fed with high fat diet and injected with dehydroepiandrosterone.After 9 weeks of breeding,metabolic parameters,the morphologic changes in ovary and expression of testosterone converting enzymes in adipose
tissue of two group were analyzed.Results Compared with group A,fasting blood glucose,insulin,testosterone,and homeostasis model assessment for insulin resistance in group B were increased (P＜0.05).All of rats in group B had lost the regular estrous cycle,and their ovaries showed polycystic changes.The expression of 3βHSD in subcutaneous and parametrial adipose tissue was increased and that of 17βHSD1 was decreased in group B compared with group A,and the expression of 5α-reductase 1 in parametrial adipose tissue was also decreased in group B (P＜0.05).Conclusions The established PCOS rat model shows insulin resistance and a variety of metabolic abnormalities.The expression of testosterone converting enzymes in adipose tissue of this model,maybe affected by the changed level of sex steroid. 【总页数】6页(216-221)
【关键词】多囊卵巢综合征;大鼠模型;脂肪组织;雄激素转换酶 【作者】王丽华;李圣贤;岳江;格日乐;韩婷婷;姚菊芳;郑璐莹;刘伟 【作者单位】200127 上海交通大学医学院附属仁济医院内分泌科 【正文语种】中文 【中图分类】 【文献来源】
https://www.zhangqiaokeyan.com/academic-journal-cn_chinese-journal-endocrinology-metabolism_thesis/0201232523752.html 【相关文献】
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多囊卵巢综合征大鼠模型脂肪组织雄激素转换酶的表达研究
