Underlying mechanism of protection from hypoxic injury seen with n-butanol extract of Pote

Underlying mechanism of protection from hypoxic injury seen with n-butanol extract of Potentilla

anserine L. in hippocampal neurons

Xiaojing Qin;Lingzhi Li;Qi Lv;Baoguo Yu;Shuwang Yang;Tao He;Yongliang Zhang

【期刊名称】《中国神经再生研究（英文版）》 【年(卷),期】2012(007)033

【摘要】The alcohol and n-butanol extract of Potentilla anserine L.significantly

protects

myocardium

from

acute

ischemic

injury.However,its effects on rat hippocampal neurons and the mechanism of protection remain unclear.In this study,primary cultured hippocampal neurons from neonatal rats were incubated in 95％ N2 and 5％ CO2 for 4 hours.Results indicated that hypoxic injury decreased the viability of neurons,increased the expression levels of caspase-9 and caspase-3 mRNA,as well as cytochrome c,Caspase-9,and Caspase-3 protein.Pretreatment with 0.25,0.0625,0.0156 mg/mL n-butanol extract of Potentilla anserine L.led to a significant increase in cell viability.Expression levels of caspase-9 and caspase-3 mRNA,as well as cytochrome c,Caspase-9,andCaspase-3 protein,were attenuated.The neuroprotective effect of n-butanol extract of Potentillaanserine L.was equivalent to tanshinone IIA.Our data suggest that the n-butanol extract of Potentilla anserine L.could protect primary hippocampal neurons from hypoxic injury by deactivating mitochondrial cell death.