HPLC法测定人工牛黄甲硝唑胶囊中甲硝唑的含量

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ＨＰＬＣ法测定人工牛黄甲硝唑胶囊中甲硝唑的含量

作者：吴卫涛

来源：《中国实用医药》2009年第09期

【摘要】 目的 建立HPLC法测定人工牛黄甲硝唑胶囊中甲硝唑的含量。方法 采用依利特C18色谱柱，甲醇-水(20∶80) 为流动相，流速：1.0 ml/min；检测波长318 nm，柱温为30℃。结果 甲硝唑在0.025~0.150 mg/ml浓度范围内呈良好线性(r=0.999 9)，平均加样回收率(n=6)为100.67%，RSD为0.33%。结论 该方法准确、可靠，与标准法比较，结果较为满意，可用于人工牛黄甲硝唑胶囊中甲硝唑的含量测定。

【关键词】HPLC法；人工牛黄甲硝唑胶囊；甲硝唑；含量测定

Determination of metronidazole in galculus bovis and metronidazole capsules by HPLC WU Wei-tao.Anyang Institute for Food and Drug Control，Anyang，Henan 455000，China

【Abstract】 Objective To establish a HPLC method for determination of metronidazole in galculus bovis and metronidazole capsules.Methods Elite C18 column was used with a mobile phase composed of methanol-water (20∶80) at a flow rate of 1.0 ml/min.The detection wavelength was 318nm and the temperature of column was 30℃.Results The calibration curve of metronidazole was linear in the range of 0.025~0.150 mg/ml，r=0.999 9.The average recovery rate was

100.67%,RSD=0.33%(n=6).Conclusion This method is accurate，realiable and can be used for determination of metronidazole in galculus bovis and metronidazole capsules.

【Key words】HPLC；Galculus bovis and metronidazole capsules；Metronidazole；Content determination

人工牛黄甲硝唑胶囊为复方制剂，是临床常用的一种抗厌氧菌药，主要用于急性智齿冠周炎、局部牙槽脓肿、牙髓炎、根尖周炎等的治疗。国家标准［1］采用紫外分光光度法测定其含量,该方法影响结果因素较多。本文介绍的高效液相色谱法,采用优化的色谱条件,使甲硝唑的含量测定操作简便,专属性高,重现性好,可以替代紫外分光光度法。