胃泌素促进人脐静脉血管内皮细胞增殖和管状结构生成
王冬梅;周建奖;谢渊;赵艳
【期刊名称】《中华内分泌代谢杂志》 【年(卷),期】2013(029)007
【摘要】Objective To study the effect of gastrin on the proliferation and angiogenesis of human umbilical vascular endothelial (HUVE) cell in vitro.Methods
The
immunocytochemistry
assay,realtime-PCR,and
Western blot were used to detect the gastrin receptor (CCK-BR) expression in HUVE cells.After HUVE cells were treated with 10 and 100 nmol/L gastrin for 72 h,MTT and soft agar colony formation assay were used to test the cell proliferation rate and colony formation rate,and the vascular-like structures were observed by three-dimensional culture of HUVE cells.The half-ring and ring vascular numbers were counted with five random visions.The vascular endothelial growth factor (VEGF) and hypoxia-inducible factor-1a (HIF-1a) mRNA and protein levels in HUVE cells were detected by realtime PCR and ELISA assay respectively.Results HUVE cells expressed CCK-BR.After the treatment with 10 and 100 nmol/L gastrin,the cell proliferation rate was increased by 48.48％ and 82.82 ％ compared to the control group,and colony formation rate was increased to 31.33％ and 45.67 ％ as compared with 15.33％ in the control group(P＜0.01).Relative expression quantities of VEGF and HIF-1a genes were 2.3 and 4.6 folds (VEGF) and 20.76 and 26.77 folds (HIF-1
a) than those in the control group.The concentration of VEGF protein in culture medium was 221 and 392 μg/mg protein higher than that in control group.The numbers of half-ring and ring vascular structures were (14.00 ± 3.00,39.33 ± 7.57 and 34.33 ± 4.50)/vision and (8.33 ± 2.51,41.33 ± 5.85 and 37.67 ± 3.51)/vision in control,10 and 100 nmol/L gastrin-treated groups,respectively (P＜0.01).Conclusion Gastrin up-regulates the expression of VEGF and HIF-1a genes in HUVE cells and promotes cell proliferation and vascular-like structure formation of HUVE cells in vitro by being combined to CCK-BR,which may be involved in the development and metastasis of gastric cancer.%目的 研究胃泌素对人脐静脉血管内皮细胞(HUVEC)体外增殖和管状结构形成的作用.方法 免疫细胞化学等检测HUVEC中胃泌素受体(CCK-BR)的表达;用10和100 nmol/L胃泌素处理HUVEC后,MTT和软琼脂克隆形成实验检测细胞增殖能力,小管形成实验观察HUVEC管状结构生成情况；实时荧光定量PCR和酶联免疫吸附法检测HUVEC中血管内皮生长因子(VEGF)和低氧诱导因子-1a(HIF-1a)基因的表达.结果 HUVEC表达CCK-BR,经10和100nmol/L胃泌素处理后,细胞增殖率和克隆形成率显著增加(P＜0.01),细胞中VEGF和HIF-1a基因的表达量高于对照组(P＜0.01),HUVEC形成的半环状和环状管状结构数也高于对照组(P＜0.01).结论 胃泌素与HUVEC表达的CCK-BR结合后,上调VEGF和HIF-1a基因的表达,促进HUVEC体外增殖及管状结构的形成能力. 【总页数】5页(598-602)
【关键词】胃泌素;血管生成;血管内皮生长因子;低氧诱导因子-1a
【作者】王冬梅;周建奖;谢渊;赵艳
【作者单位】成都市第十人民医院检验科;550004 贵阳医学院分子生物学重点实验室;贵阳医学院附属医院检验科;550004 贵阳医学院分子生物学重点实验室;550004 贵阳医学院分子生物学重点实验室 【正文语种】中文 【中图分类】 【文献来源】
https://www.zhangqiaokeyan.com/academic-journal-cn_chinese-journal-endocrinology-metabolism_thesis/0201232527639.html 【相关文献】
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胃泌素促进人脐静脉血管内皮细胞增殖和管状结构生成
