牛羊肉中肠出血性大肠杆菌O157∶H7的检测
王晓红;应兰;李增魁
【期刊名称】《安徽农业科学》 【年(卷),期】2012(040)032
【摘要】[目的]为青海省部分地区市售的羊肉、牛肉进行了大肠杆菌O157∶H7的检测.[方法]样品先用mEC肉汤增菌,然后在选择性培养基TC-SMAC培养,再根据GenBank上已发表的大肠杆菌O157∶H7 rfbE、fliC 和 eacA 抗原基因的保守序列设计3对引物,进行多重PCR鉴定,将可疑菌接种于MUG-LST,进行微量生化试验鉴定.[结果]在各1株牛、羊肉分离菌中多重PCR同时扩增出了大小为678、560和368 bp的3条特异性条带,分离菌的生化反应特性符合大肠杆菌的特征.[结论]在羊、牛肉各1份样品中检出了E.coliO157∶H7.%[Objective] To detect the Escherchia coli O157: H7 from beef and mutton in Qinghai. [Method] The collected samples were culturedrnin mEC + n broth, at 41 °C for 24 hours, the enriched cultures were cultivated on TC-SMAC plates at 37 °C for another 24 hours and 3 pairs ofrnprimers were designed based on the rfbE, fliC and eacA genes of E. coli O157: H7 in GenBank and multiplex PCR were established specific tornrfbE, fliC and eacA gene. The suspicious bacterial were cultivated on MUG-LST and preliminarily identified by micro-biochemical reactions.rn[Result] Fragments of 678 bp(rfbE), 560 bp(fliC) and 368 bp(eacA) could be amplified from beef and mutton isolated bacteria respectivelyrnand its micro-biochemical reactions
牛羊肉中肠出血性大肠杆菌O157∶H7的检测
