突变型低氧诱导因子-1α修饰骨髓间充质干细胞来源的外
泌体联合软骨再生支架治疗家兔早期软骨缺损
肖大伟;刘丹平;田大川;周山健;李海乐;綦惠
【期刊名称】《中华实验外科杂志》 【年(卷),期】2024(035)005
【摘要】Objective Cartilage defects is one of the common joint diseases throughout the world.We hypothesized that exosomes may have supporting role in treating this disease.Methods In vitro,bone marrow mesenchymal stem cells (BMSCs) were transfected with or without mutant hypoxia inducible factor (HIF)-1α.Exosomes derived from both kinds of BMSCs (BMSC-Exonor or BMSC-Exomu) were isolated and identified.Chondrocytes were treated with tumor necrosis factor (TNF)-α,and BMSC-Exonor or BMSC-Exomu were also added at the same time.Proliferation,apoptosis,and the activity of mitogen-activated protein kinases (MAPKs) and protein kinase B (Akt) were examined.In vivo,BMSC-Exonor or BMSC-Exomu were combined with porous scaffold to repair cartilage defects,and the efficacy was assessed by histological examination.Results MTT assay and Western blot results showed that BMSC-Exonor could play a role in TNF-α-mediated chondrocyte apoptosis,the difference was statistically significant (P =0.006),while BMSC-Exomu was more effective (P =0.001).While in vivo,BMSC-Exomu improved regeneration of early cartilage defects of
突变型低氧诱导因子-1α修饰骨髓间充质干细胞来源的外泌体联合软
