Biochemical pathways in the antiatherosclerotic
effect of berberine
GUO Yi;WANG Qi-zhang;LI Fang-ming;JIANG Xin;ZUO Yan-fang;WANG Ling
【期刊名称】《中华医学杂志（英文版）》 【年(卷),期】2008(121)013
【摘要】Background This study investigated the inhibitory effect of berberine(BBR)on 2(COX-2)expression
lipopolysaccharide(LPS)induced
via
the
mitogen
cyclooxygenase-protein
activated
kinase(MAPK)signalling cascade pathways in human peripheral blood monocytes(PBMC).Methods PBMC from whole blood were isolated and cultured for uD 10 24 hours after division into 5 groups treated with LPS,LPS+BBR 25 μmol/L,LPS+BBR 50 μmol/L or LPS+BBR 100 μmol/L and untreated.Monocytes were extracted for RT-PCR and Western blot analyses to examine COX-2 mRNA and protein activated expression of p38 mitogen activated protein kinase(p38MAPK),Jun N-terminal kinase(JNK)and extracellular regulated kinases 1/2(ERK1/2)signalling pathways.Results COX-2 mRNA and protein expression decreased to a minimum at 12 hours after BBR treatment fP＜0.05).With the increasing concentration of BBR treatment,the COX-2 expression decreased progressively(P＜0.01).With BBR treatment for 6,12 or 24 hours at three doses,ERK1/2 protein expression was significantly inhibited.For the JNK pathway,only with the treatment of BBR at the concentration of 100
Biochemical pathways in the antiatherosclerotic effect of berberine
