益肾胶囊对大鼠肾小球系膜细胞增殖和凋亡及TGF-β1mRNA表达的影响
吴喜利;孙万森;石兴民;安鹏;乔成林;王竹
【期刊名称】《中国中西医结合肾病杂志》 【年(卷),期】2011(012)006
【摘要】目的:观察益肾胶囊对大鼠肾小球系膜细胞(glomerular mesangial cell,GMC)增殖、凋亡及TGF-β1mRNA表达的影响.方法:以体外培养肾小球系膜细胞为研究对象,应用血清药理学方法,分别采用甲基噻唑基四唑(methyl thiazolyl tetrazolium,MTT)比色法、荧光倒置显微镜及RT-PCR技术检测系膜细胞增殖、凋亡及TGF-β1mRNA表达情况.结果:与正常对照组比较,其他各组MC均有不同程度的增殖、凋亡及TGF-β1 mRNA表达上调(P<0.05或P<0.01);与脂多糖(lipopolysaccharides,LPS)组比较,益肾胶囊可明显抑制MC增殖及TGF-β1 mRNA表达,并诱导MC凋亡(P<0.01).结论:益肾胶囊抑制MC增殖并诱导其凋亡可能与下调TGF-β1 mRNA表达有关.%Objective:To investigate the effects of yishen capsule on the proliferation, apoptosis and expression of TGF - β1 mRNA of rat glomerular mesangial cell ( GMC ).Methods : Utilizing the method of serum pharmacology to study the cultured GMC,and the proliferation, apoptosis and expression of TGF - β1 mRNA of GMC were detected by methyl thiazolyl tetrazolium ( MTT ) , fluorescence inverted microscope and the technique method respectively.Results : Compared with control group, the proliferation, apoptosis and TGF - β1 mRNA expression of GMC of
益肾胶囊对大鼠肾小球系膜细胞增殖和凋亡及TGF-β1mRNA表达的影响
