低能离子注入诱变选育盐霉素高产菌株
武利勤;苗凤香;顾海科;尚宏忠
【期刊名称】《安徽农业科学》 【年(卷),期】2012(040)028
【摘要】[目的]研究N+离子注入对白色链霉菌的诱变效应,同时筛选高产盐霉素的变异菌株.[方法]利用不同剂量的氮离子对白色链霉菌S-11-04菌株进行诱变处理,研究低能氮离子注入对其存活率、菌落形状及产盐霉素能力的影响.[结果]低能氮离子注入对白色链霉菌的诱变效应显著,试验得到了13株盐霉素产量较高的突变菌株,其中N3-6菌株经连续传代4次,遗传稳定性较好,其摇瓶发酵水平较对照提高了41％,发酵生产后平均发酵水平提高了20.5％.[结论]离子注入诱变是获得高产盐霉素突变菌株的有效方法.%[ Objective] This study aimed to explore the mutagenesis effects of N+ ion beam irradiation on Streptomyces albus and obtain high-yield salinomycin-producing strain. [Method] Streptomyces albus strain S-ll-04 was mutated with different doses of N+ implantation. The effects of low energy N+ implantation on the survival rate, colony shape and salinomycin-producing ability were investigated. [ Result] The results showed that low energy N+ implantation can efficiently improve the positive mutation rate of Streptomyces albus; 13 mutant strains with high yield of salinomycin were isolated; mutant strain N3-6 has good inheritance and fermentation stability with four continuous generations, and the litres of salinomycin were increased by 41% in the shake-flask cukure and 20. 5%
in mass production compared with the control. [Conclusion] N+ ion beam irradiation is an effective method to obtain high-yield salinomycin-producing Streptomyces albus strain. 【总页数】3页(13734-13735,13764)
【关键词】白色链霉菌;氮离子注入;盐霉素;诱变育种 【作者】武利勤;苗凤香;顾海科;尚宏忠
【作者单位】北京市辐射中心,北京100875;鲁抗菏泽舍里乐药业有限公司,山东菏泽274016;北京市辐射中心,北京100875;北京市辐射中心,北京100875 【正文语种】中文 【中图分类】S188 【文献来源】
https://www.zhangqiaokeyan.com/academic-journal-cn_journal-anhui-agricultural-sciences_thesis/020121847987.html 【相关文献】
1.低能离子注入诱变美伐他汀高产菌株的选育 [J], 张莉; 刘靓; 张玉莲; 陈丽华 2.低能N+离子注入诱变选育云芝高产抗病菌株的研究 [J], 蒋益; 郑惠华; 刘广建; 全卫丰; 薛璟; 季宏更; 汪洁
3.氮离子注入诱变选育恩拉霉素高产菌株 [J], 蔡作新; 郑传伟; 田伟波; 魏亚萍; 艾咚咚
4.低能离子注入在CGTase高产菌株选育中的应用 [J], 王雁萍; 段宇珩; 许平辉; 秦广雍; 霍裕平
5.离子注入结合紫外线及60Co-γ射线诱变选育碱性果胶酸裂解酶高产菌株的
研究 [J], 雷建湘; 刘贤雪; 沈雪亮; 汪钊
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低能离子注入诱变选育盐霉素高产菌株
