右美托咪定对脂多糖诱导单核巨噬细胞炎症反应的影响
陆季娟;杭黎华
【期刊名称】《国际麻醉学与复苏杂志》 【年(卷),期】2018(039)002
【摘要】Objective To explore the effect of dexmedetomidine (Dex) on the release of cell cytokines from monocyte macrophage RAW264.7 cells induced by lipopolysaccharide (LPS) and the expression of heme oxygenase (HO)-1. Methods RAW264.7 cells were cultured in six-well culture dishes with a density of 1×106/ml(2 ml/hole)and were randomly divided into 5 groups(n=6)by the number table method:normal control group(C group),Dex 10 μg/L(Dex group),LPS 1 mg/L(LPS group),LPS 1 mg/L+Dex 10 μg/L (LPS+Dex group),and ZnPPⅨ (inhibitor of HO-1),10 μmol/L+LPS 1 mg/L+Dex,10 μg/L (ZnPPⅨ+LPS+Dex group). After incubation of 3, 6, 12, 24 h, ELISA was used to determine the concentrations of TNF-α, IL-6 and high mobility group box-1 protein(HMGB-1)in the supernatants of six wells in each group.Then,six wells in each group were chosen at incubation of 6 h and 12 h for determination of HO-1 expression by Western blot. Results Comparing with C group, the concentrations of TNF-α, IL-6 and HMGB-1 in the supernatants at 3,6,12,24 h in the LPS group were significantly increased (P<0.05),and the expression of HO-1 at 6 h and 12 h time point was up-regulated in LPS group. Compared with LPS group, the concentrations
of TNF-α, IL-6 and HMGB-1 in LPS+Dex group were
attenuated(P<0.05).Meanwhile,the expression of HO-1 at 6 h and 12 h time point was up-regulated in LPS+Dex group.Compared with LPS+Dex group,the concentrations of TNF-α,IL-6 and HMGB-1 in ZnPPⅨ+LPS+Dex group were increased (P<0.05). However,the expression of HO-1 was reduced in ZnPPⅨ+LPS+Dex group (P<0.05). Conclusions Dex could inhibit the release of cell cytokines from RAW264.7 via up-regulating the levels of HO-1 expression.%目的 探讨右美托咪定(dexmedetomidine,Dex)对脂多糖(lipopolysaccharides,LPS)诱导单核巨噬细胞RAW264.7炎性因子释放及血红素氧合酶(heme oxygenase,HO)-1表达的影响. 方法 离体培养小鼠单核巨噬细胞系RAW264.7,用含有1×106个/ml的细胞样本接种于6孔培养板(2 ml/孔).采用随机数字表法将其分为5组(每组6孔):正常对照组(C组)、Dex 10 μg/L组(Dex组)、LPS 1 mg/L组(LPS组)、LPS 1 mg/L+Dex 10 μg/L组(LPS+Dex组)及HO-1抑制剂ZnPPⅨ10 μmol/L+LPS 1 mg/L+Dex 10 μg/L组(ZnPPⅨ+LPS+Dex组).分别于孵育3、6、12、24 h时点收集细胞培养上清液,采用ELISA法测定TNF-α、IL-6、高迁移率族蛋白1(high mobility group box-1 protein,HMGB-1)的浓度;在6 h和12 h时点收集细胞沉淀,提取蛋白成分,利用Western blot法测定HO-1表达. 结果 与C组比较,LPS组各时间点TNF-α、IL-6、HMGB-1浓度均明显升高(P<0.05),在6、12 h时点细胞HO-1的表达上调(P<0.05);与LPS组比较,LPS+Dex组各时间点的TNF-α、IL-6、HMGB-1浓度降低(P<0.05),培养细胞6、12 h时点HO-1表达升高;与LPS+Dex组比较,ZnPPⅨ+ LPS+Dex组各
时间点的TNF-α、IL-6、HMGB-1浓度升高(P<0.05),培养细胞6、12 h时点HO-1表达降低(P<0.05). 结论 Dex可以抑制LPS诱导的单核巨噬细胞炎症因子释放,其机制与上调HO-1表达有关. 【总页数】4页(136-138,169)
【关键词】右美托咪定;炎症因子;脂多糖;血红素氧合酶-1 【作者】陆季娟;杭黎华
【作者单位】215300,江苏大学附属昆山第一人民医院麻醉科;215300,江苏大学附属昆山第一人民医院麻醉科 【正文语种】中文 【中图分类】 【文献来源】
https://www.zhangqiaokeyan.com/academic-journal-cn_international-journal-anesthesiology-resuscitation_thesis/0201233098407.html 【相关文献】
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右美托咪定对脂多糖诱导单核巨噬细胞炎症反应的影响
