电刺激大鼠小脑顶核对缺血-再灌注视网膜的保护作用实验
研究
罗哲文;张虹
【期刊名称】《昆明医科大学学报》 【年(卷),期】2011(032)010
【摘要】Objective To investigate the protective effect and mechanism of electrical stimulating cerebellar fastigial nucleus on retina against ischemia/reperfusion injury in rats. Methods Rats were divided randomly into three groupsrgroupl ( ischemia-reperfusion) , group2 ( electrical-stimulating-treated) , and group3 (sham-operated). Apoptosis of retinal ganglion cells was detected by TUNEL method, and expression of Bcl-2 and Bax were measured by immunohistochemical staining methods. Results Ischemia/reperfusion injury induced the apoptosis of retinal ganglion cells (P<0.05), meanwhile, as ischemia prolonged, the expression of Bcl-2 became weaker, and the expression of Bax became stronger gradually (P<0.05). Electrical stimulating cerebellar fastigial nucleus alleviated these changes in treated group (P <0.05). Conclusions Electrical stimulating cerebellar fastigial nucleus can protect retina against ischemia/reperfusion-injury. The possible mechanism includes upregulating the protein expression of Bcl-2 and downregualting the protein expression of Bax.%目的 探讨电刺激大鼠小脑顶核(cerebellar fastigial nucleus,CFN)对视网膜缺血-再灌注损伤的保护作用
电刺激大鼠小脑顶核对缺血-再灌注视网膜的保护作用实验研究
