Immunological Evaluation of a Novel Mycobacterium tuberculosis Antigen Rv0674

Immunological Evaluation of a Novel Mycobacterium tuberculosis Antigen Rv0674

XIAO Tong Yang;LI Ma Chao;WAN Kang Lin;LIU Hai Can;LI Xiao Qin;HUANG Ming Xiang;LI Gui Lian;LI Na;YAN Yu Han;LUO Qiao;WANG Xue Zhi

【期刊名称】《生物医学与环境科学（英文版）》 【年(卷),期】2019(032)006

【摘要】Objective This study aimed to characterize the diagnostic and vaccine potential of a novel Mycobacterium tuberculosisantigen Rv0674. Methods To evaluate thediagnostic potential and antigenicity of Rv0674, IgG was evaluated using ELISA and interferon (IFN)-γ was done by using ELISpot assay among TB patients and healthy donors. For immunogenicity evaluation, BALB/c mice were immunized with Rv0674. Cytokine production was determined by cytokine release assay using an ELISA kit, and the antibodies were tested using ELISA. Results The results

of

serum

Elisa

tests

showed

that

Rv0674

specific

immunoglobulin G (IgG) response was higher in TB patients than negative controls. And Rv0674 had good performance in serological test with sensitivity and specificity of 77.1％ and 81.1％, respectively. While it shows poor sensitivity and specificity of 26.23％ and 79.69％ for IFN-γ tests. In BALB/c mice, Rv0674 adjuvant by DDA/PolyI:C could also induce a high level of IFN-γ, interleukin-2 and interleukin-6 as well as a high IgG titer in both high-and low-dose groups indicating that Rv0674