Evaluation of anti-apoptotic activity of different dietary antioxidants in renal cell carc

Evaluation of anti-apoptotic activity of different dietary antioxidants in renal cell carcinoma against

hydrogen peroxide

Garg;Neeraj;K;Mangal;Sharad;Sahu;Tejram;Mehta;Abhinav;Vyas;Suresh;P;Tyagi;Rajeev;K;;;;;;;;

【期刊名称】《亚太热带生物医学杂志：英文版》 【年(卷),期】2011(000)001

【摘要】Objective:To evaluate the anti-apoptotic and radical scavenging activities of dietary phenolics, namely ascorbic acid,a-tocopherol acetate,citric

acid,salicylic

acid,and

estimate

H₂O₂induced apoptosis in renal cell carcinoma cells.Methods:The intracellular antioxidant potency of antioxidants was investigated.H₂O-2-induced apoptosis in RCC-26 was assayed with the following parameters:cell viability（%apoptosis）,nucleosomal damage and DNA fragmentation, bcl-2 levels

and

flow

cytometery

analysis

（

ROS

production

evaluation）.Results:Ine anticancer properties of antioxidants such as ascorbic acid,a- tocopherol acetate,citric acid,salicylic acid with perdurable responses were investigated.It was observed that these antioxidants had protective effect（anti-apoptotic activity） against hydrogen peroxide（H₂O₂） in renal cell carcinoma（RCC-26） cell line.Conclusions:This study reveals and