基于SwissPdbViewer和MATLAB对东北虎Sox蛋白三级结构建模及分析

基于SwissPdbViewer和MATLAB对东北虎Sox蛋白三

级结构建模及分析

梁俊毅;陈启龙

【期刊名称】《四川动物》 【年(卷),期】2008(027)006

【摘要】Sox proteins are characterized by a conserved hiigh mobility group HMG-box domain,which is responsible for DNA binding andbonding.In order to research the molecular mechanism of Sox protein in Panthera tigris altaica,the amino acid sequences of PtSox were downloaded from the C,enBank database using bioinformatics tools of MATLAB.The tertiary structures of PtSox proteins were modeled using computer programs SwissPdbViewer and MATLAB,homology modeling method,and the known tertiary structure of Sox2 as templates.The three-dimensional structures of predicted PtSox proteins were visualized using Visualization Tool of MATLAB.The results showed that the tertiary structure of PtSox appeared to three α-helices and two loop regions.Thermestability analysis showed that the loop regions of PtSox structures were relatively unstable thermodynamically.Analysis of the electrostatic surfaces showed the C-terminal half of PtSox formed an N/C cavity where interacted with organic molecules or proteins could be possible.This observation suggested that the spatial structure of Sox was more likely to regulate the activity and function of this