文多灵对大鼠肾血管的舒张作用
李勇;邓丽娟;武小林;杨小林;叶文才;张冬梅
【期刊名称】《中国药理学通报》 【年(卷),期】2012(028)008
【摘要】目的 研究文多灵对大鼠肾血管的舒张效应及作用机制.方法 快速分离大鼠肾动脉并固定于多通道微血管肌动扫描仪中,预孵育不同的受体抑制剂或离子通道阻断剂,记录给药后血管环等张力的变化.结果 文多灵对由苯肾上腺素、KCl预收缩的血管呈剂量依赖性地舒张;四乙铵(TEA+)、Ro-32-0432能部分拮抗文多灵的舒张血管效应,而格列本脲、BaCl2或Y-27632对其舒张作用没有明显的影响;文多灵在无钙的高钾溶液中能浓度依赖性地抑制CaCl2的血管收缩作用以及舒张由(-)Bay K8644预收缩的血管.此外,与文多灵舒血管效应与内皮细胞无关.结论 文多灵在体外可能通过抑制血管平滑肌细胞外钙内流、TEA+-敏感的钾离子通道或蛋白激酶C途径舒张大鼠肾血管.%Aim To explore the underlying relaxation mechanisms of vindoline in isolated rat renal arteries. Methods Rings were quickly isolated and suspended in a Mutti Myograph System and changes of isometric tension were recorded in the absence or presence of different receptor inhibitors or ion channel blockers. Results Vindoline produced a dose-dependent relaxation in rings with or without endothelia contracted by phenylephrine or KC1. Treatment with TEA+ or Ro-34-0432 slightly blunt the relaxation induced by vindoline, whereas gliclamide, BaCl2 or Y-27632 failed to affect this relaxant effect. Vindoline reduced the con-traction evoked by
文多灵对大鼠肾血管的舒张作用
