萘哌地尔衍生物YMⅢ抗AngⅡ诱导SHR血管平滑肌细胞
增殖的作用
邱敏;杨征;李利生;吴芹;金凤;黄燮南
【期刊名称】《中国药理学通报》 【年(卷),期】2012(028)009
【摘要】Aim To investigate the effect of YM M , a ramification of naftopidil, on Angiotensin Ⅱ ( Ang Ⅱ )-induced proliferation of vascular smooth muscle cell ( VSMC ) from spontaneously hypertensive rat ( SHR ) in vitro and probe preliminarily its mechanisms of action. Methods In cultured VSMCs from SHR, the changes of cell proliferation, DNA synthesis, cell cycle progress, cell ultramicrostructure and Agt, c-myc mR-NA expressions induced by Ang Ⅱ were evaluated by MTT assay,[ 3H ]-TdR incorporating method, flow cy-tometry assay, transmission electron microscopy tech-nigue and real time RT-PCR, respectively, and the influences of YM M on the above parameters were observed. Results Compared with the model controlrngroup, YMI 0. 01,0. 05 ,0. 10 μmol · L-1 could con-centration-dependently inhibit the cell prolifetation and the elevated rate of [ 3H ]-TdR incorporation induced by Ang Ⅱ 1μmol · L-1; this agent could also block the cell cycle progress, improve the cell ultramicrostructure, and down-regulate the elevated Agt and c-myc mRNA expressions induced by Ang Ⅱ. Conclusion YM M has an inhibitory effect on the proliferation of SHR
萘哌地尔衍生物YMⅢ抗AngⅡ诱导SHR血管平滑肌细胞增殖的作用
